Antibody:	Anti-Sox9 (AB5535)
Detection system:	horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	1A Sox9	Expression in cartilage primordium of forelimb bud mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: hindlimb pre-cartilage condensation	Present		1B Sox9	Expression in cartilage primordium of hind limb bud.

Structure	Level	Pattern	Image	Note
TS21: femur cartilage condensation	Present	Widespread	1C Sox9	Expression throughout entire cartilage element.

Structure	Level	Pattern	Image	Note
TS22: femur cartilage condensation	Present	Regionally restricted	1D Sox9	Expression in enttire cartilage element, with strongest expression in central region.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: femur		Present	Regionally restricted	1E Sox9	Expression in femoral growth plate cartilage.
TS23: epiphyseal plate	growth plate cartilage chondrocyte	Strong	Regionally restricted	1E Sox9	Abundant expression in prehypertrophic chondrocytes.
TS23: epiphyseal plate proliferative zone	growth plate cartilage chondrocyte	Strong		1E Sox9	Abundant expression in proliferating zone and resting zone chondrocytes.
TS23: epiphyseal plate resting zone	growth plate cartilage chondrocyte	Strong		1E Sox9	Abundant expression in proliferating zone and resting zone chondrocytes.
TS23: epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Weak		1E Sox9	Decreased expression in hypertrophic chondrocytes.