Gene Expression Data
Assay Details
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Assay
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Reference:
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J:314133
Marczenke M, et al., GAS1 is required for NOTCH-dependent facilitation of SHH signaling in the ventral forebrain neuroepithelium. Development. 2021 Nov 1;148(21):dev200080
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6866994
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Gene symbol:
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Hes5
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Gene name:
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hes family bHLH transcription factor 5
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Probe:
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Mm-Hes5 (400991)
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Probe preparation:
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Antisense
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Visualized with:
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Fluorescein
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Assay notes:
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Two Hes5 probes were described, but the one used in this assay was not detailed. Hes5 probe20 MGI:6866980 may have been used in this assay.
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Results
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Specimen
3A Hes5 Control:
embryonic day 8.5
(more  )
Note: 8-9 somites
Specimen
3A Hes5 Control:
(close  )
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Genetic Background:
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involves: C57BL/6N
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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8-9 somites
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Specimen
3A Hes5 Gas1-/-:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Gas1tm2Fan/Gas1tm2Fan
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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8-9 somites
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Specimen
3B Hes5 Control:
embryonic day 8.5
(more )
Note: 10-11 somites
Specimen
3B Hes5 Control:
(close )
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Genetic Background:
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involves: C57BL/6N
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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10-11 somites
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Specimen
3B Hes5 Gas1-/-:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Gas1tm2Fan/Gas1tm2Fan
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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10-11 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future prosencephalon |
Weak |
Regionally restricted |
3B Hes5 Gas1-/-
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As early as E8.5, preceding defect in Shh pathway reduced expression in rostral neuroepithelium compared with controls. .
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Specimen
3C Hes5 Control:
embryonic day 9.5
(more )
Specimen
3C Hes5 Control:
(close )
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Genetic Background:
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involves: C57BL/6N
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3C Hes5 Gas1-/-:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Gas1tm2Fan/Gas1tm2Fan
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Weak |
Regionally restricted |
3C Hes5 Gas1-/-
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Expression in rostral ventral neuroepithelium further reduced compared with controls.
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Specimen
3D Hes5 Control:
embryonic day 10.5
(more )
Specimen
3D Hes5 Control:
(close )
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Genetic Background:
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involves: C57BL/6N
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3D Hes5 Gas1-/-:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Gas1tm2Fan/Gas1tm2Fan
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forebrain |
Weak |
Regionally restricted |
3D Hes5 Gas1-/-
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Expression in rostral ventral neuroepithelium further reduced compared with controls.
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Specimen
S4B Hes5 Control:
embryonic day 10.5
(more )
Specimen
S4B Hes5 Control:
(close )
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Genetic Background:
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involves: C57BL/6N
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
S4B Hes5 Gas1-/-:
(close )
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Genetic Background:
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involves: C57BL/6N
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Mutant Allele(s):
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Gas1tm2Fan/Gas1tm2Fan
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools