Gene Expression Data
Assay Details
Assay
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Reference:
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J:61134
Eichmann A, et al., Developmental expression of pim kinases suggests functions also outside of the hematopoietic system. Oncogene. 2000 Feb 24;19(9):1215-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6866973
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Gene symbol:
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Pim1
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Gene name:
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proviral integration site 1
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Probe:
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Pim1 cDNA5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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The fixative was Serra's solution (60% ethanol, 30% formaldehyde, 10% acetic acid).
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Results
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Specimen
6A:
embryonic day 10.0
(more )
Specimen
6A:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS16: yolk sac mesenchyme |
hematopoietic cell |
Strong |
Regionally restricted |
6A
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Expression was detected in the majority of hematopoietic cells of the blood islands.
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Specimen
6B:
embryonic day 10.0
(more )
Specimen
6B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS16: yolk sac blood vessel |
hematopoietic cell |
Present |
|
6B
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Expression was detected in the majority of hematopoietic cells of the more mature vessels of the yolk sac.
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Specimen
not shown E10:
embryonic day 10.0
(more )
Specimen
not shown E10:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
not shown E14:
embryonic day 14.0
(more )
Specimen
not shown E14:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
|
Paraffin
|
Specimen
6C:
embryonic day 17.0
(more )
Specimen
6C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Specimen
not shown E17:
embryonic day 17.0
(more )
Specimen
not shown E17:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
|
Paraffin
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Specimen
7C:
embryonic day 17.0
(more )
Specimen
7C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS25: thymus |
Present |
Regionally restricted |
7C
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Scattered positively stained cells were observed in the medullary region.
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Specimen
8A:
embryonic day 17.0
(more )
Specimen
8A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Paraffin
|
Specimen
8B:
embryonic day 17.0
(more )
Specimen
8B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Specimen
8C:
embryonic day 17.0
(more )
Specimen
8C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Paraffin
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Specimen
9A:
embryonic day 17.0
(more )
Specimen
9A:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS25: olfactory epithelium |
neuron |
Strong |
Regionally restricted |
9A
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Expression was localized in the intermediate layer of differentiating neurons, as well as in the layer of more mature neurons.
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Specimen
9D:
embryonic day 17.0
(more )
Specimen
9D:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS25: olfactory epithelium |
neuron |
Strong |
Regionally restricted |
9D
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Expression was localized in the intermediate layer of differentiating neurons, as well as in the layer of more mature neurons.
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Specimen
9G:
embryonic day 17.0
(more )
Specimen
9G:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Paraffin
|
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