Nkx2-1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:243650 Herriges MJ, et al., The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017 May 01;31(9):889-903
Assay type:	RT-PCR
MGI Accession ID:	MGI:6860418
Gene symbol:	Nkx2-1
Gene name:	NK2 homeobox 1

Probe:	Nkx2.1-pA, Nkx2.1-pB
Probe preparation:	labelled with SYBR green

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
Wild-type	Fractionated sample: no data stored
NANCI^{creERT2-RFP/+}	Fractionated sample: no data stored
Nkx2.1^GFP/+	Fractionated sample: no data stored
NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+	Fractionated sample: no data stored
Wild-type	P w 10	TS28: lung	Present	Not Specified; total RNA	Not Specified		Not Specified
NANCI^{creERT2-RFP/+}	P w 10	TS28: lung	Present (a)	Not Specified; total RNA	Not Specified	Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺	Not Specified
Nkx2.1^GFP/+	P w 10	TS28: lung	Present (b)	Not Specified; total RNA	involves: 129S6/SvEvTac	Nkx2-1^tm1Ikono/Nkx2-1⁺	Not Specified
NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+	P w 10	TS28: lung	Present (c)	Not Specified; total RNA	involves: 129S6/SvEvTac	Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺	Not Specified
Wild-type	P m 6	TS28: lung	Present	Not Specified; total RNA	Not Specified		Not Specified
NANCI^{creERT2-RFP/+}	P m 6	TS28: lung	Present (a)	Not Specified; total RNA	Not Specified	Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺	Not Specified
Nkx2.1^GFP/+	P m 6	TS28: lung	Present (b)	Not Specified; total RNA	involves: 129S6/SvEvTac	Nkx2-1^tm1Ikono/Nkx2-1⁺	Not Specified
NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+	P m 6	TS28: lung	Present (c)	Not Specified; total RNA	involves: 129S6/SvEvTac	Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺	Not Specified

Notes:
(a) Expression levels are comparable to those seen in wild type.
(b) Unlike earlier in development, expression levels are restored to those seen in wild type.
(c) Expression levels are significantly reduced in this mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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