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Gene Expression Data
Assay Details
Assay
Reference: J:133832 Festoff BW, et al., Motor neuron cell death in wobbler mutant mice follows overexpression of the G-protein-coupled, protease-activated receptor for thrombin. Mol Med. 2000 May;6(5):410-29
Assay type: RT-PCR
MGI Accession ID: MGI:6850695
Gene symbol: F2r
Gene name: coagulation factor II thrombin receptor
Probe: PAR1-pC, PAR1-pD
Visualized with: Ethidium bromide
Assay notes: Quantitative reaction using a mimic product (a subclone with a deletion introduced - pRMS11). Titrated amounts of control, mimic product into the RT reaction prior to amplification allowed the estimated copy number of transcript in the wr/wr spinal cord compared with wild type.
Results Image: 5
 Sample Information Bands Other Sample Information
Lane AgeStructure 660 bp Amount Genetic BackgroundMutant Allele(s)Sex
Wild Type cerebellum P adult TS28: cerebellum Present 1 µg; total RNA involves: NFR/N Not Specified
Wobbler cerebellum P adult TS28: cerebellum Present 1 µg; total RNA involves: NFR/N Vps54wr/Vps54wrNot Specified
Wild Type cervical SC P adult TS28: spinal cord cervical region Present 1 µg; total RNA involves: NFR/N Not Specified
Wobbler cervical SC P adult TS28: spinal cord cervical region Present (a) 1 µg; total RNA involves: NFR/N Vps54wr/Vps54wrNot Specified
Wild Type thoraco-lumbar SC P adult TS28: spinal cord lumbar region
TS28: spinal cord thoracic region 		Present 1 µg; total RNA involves: NFR/N Not Specified
Wobbler thoraco-lumbar SC P adult TS28: spinal cord lumbar region
TS28: spinal cord thoracic region 		Present 1 µg; total RNA involves: NFR/N Vps54wr/Vps54wrNot Specified
Notes:
(a) The increase in expression was greatest in the wobbler cervical spinal cord compared with both cerebellum and thoraco-lumbar spinal cord.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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