Antibody:	anti-TSPO [EPR5384] (ab109497)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Leydig cell	Leydig cell	Present		3A2 , 3A1 , 3A
TS28: Sertoli cell	Sertoli cell	Present		3A1 , 3A2 , 3A

Structure	Cell Type	Level	Pattern	Image	Note
TS22: seminiferous cord		Weak	Regionally restricted	5_E14.5' , 5_E14.5	Diffuse expression was in both gonocytes and Sertoli cells. Localization in the gonocytes was enriched in a perinuclear compartment.
TS22: Sertoli cell	Sertoli cell	Weak	Diffuse	5_E14.5' , 5_E14.5
TS22: interstitium of the testis		Strong	Regionally restricted	5_E14.5' , 5_E14.5	A few strongly positive interstitial cells were noted at E14.5.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: seminiferous cord		Weak	Regionally restricted	5_E18.5 , 5_E18.5'	Diffuse expression was in both gonocytes and Sertoli cells.
TS26: Sertoli cell	Sertoli cell	Weak	Diffuse	5_E18.5 , 5_E18.5'

Structure	Cell Type	Level	Pattern	Image	Note
TS27: seminiferous cord		Weak	Regionally restricted	5_P0 top right , 5_P0 lower right	Diffuse expression was in both gonocytes and Sertoli cells.
TS27: Sertoli cell	Sertoli cell	Weak	Diffuse	5_P0 lower right , 5_P0 top right

Structure	Cell Type	Level	Pattern	Image	Note
TS28: seminiferous cord		Present	Regionally restricted	5_P7	Diffuse expression was in both gonocytes and Sertoli cells.
TS28: Sertoli cell	Sertoli cell	Present	Diffuse	5_P7
TS28: interstitium of the testis		Strong	Regionally restricted	5_P7	A few strongly positive interstitial cells were noted.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: testis		Present		5_P14	The expression pattern was similar to the adult.
TS28: Leydig cell	Leydig cell	Present		5_P14	In the Leydig cells, expression appeared higher compared to adult.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: testis		Present		5_P21	The expression pattern was similar to the adult.
TS28: Leydig cell	Leydig cell	Present		5_P21	In the Leydig cells, expression appeared higher compared to adult.

Structure	Level	Pattern	Image	Note
TS28: epithelium of epididymis	Strong	Regionally restricted	6A	Expression was detected in the epithelium of the distal efferent ducts.

Structure	Level	Pattern	Image	Note
TS28: epithelium of caput of epididymis	Present	Regionally restricted	6B	Expression was detected in the epithelium of segment I of the caput epididymis; expression was confined to the apical and basal regions, specific to narrow and basal cells.

Structure	Level	Pattern	Image	Note
TS28: epithelium of caput of epididymis	Present	Non-Uniform	6C	Expression was detected in the epithelium of segment II of the caput epididymis. Expression was detected in all cells, but prominently higher expression was observed in the narrow cells.

Structure	Level	Pattern	Image	Note
TS28: epithelium of caput of epididymis	Present	Regionally restricted	6D	Expression was detected in the epithelium of segment III of the caput epididymis. Expression was weaker than that observed in segment II, but the levels in narrow cells and basal cells appeared unchanged.

Structure	Level	Pattern	Image	Note
TS28: epithelium of corpus epididymis	Present	Regionally restricted	6E	Expression was restricted exclusively to the basal cells and clear cells.

Structure	Level	Pattern	Image	Note
TS28: epithelium of cauda of epididymis	Present		6F	Almost all cells forming the epithelial layer appeared to show expression.

Structure	Level	Pattern	Image	Note
TS28: epithelium of seminal vesicle	Weak		7A , 7A'

Structure	Level	Pattern	Image	Note
TS28: ventral prostatic epithelium	Strong		7B , 7B'

Structure	Level	Pattern	Image	Note
TS28: dorsal prostatic epithelium	Present	Regionally restricted	7C' , 7C	Epithelium of the dorsolateral prostate showed a distinct apically polarized expression.
TS28: lateral prostatic epithelium	Present	Regionally restricted	7C' , 7C	Epithelium of the dorsolateral prostate showed a distinct apically polarized expression.

Structure	Level	Pattern	Image	Note
TS28: mesenchymal stroma of ovary	Strong		8A	Expression was detected in the interstitial cells.

Structure	Level	Pattern	Image	Note
TS28: surface epithelium of ovary	Strong		8B
TS28: mesenchymal stroma of ovary	Strong		8B	Expression was detected in the interstitial cells.

Structure	Level	Pattern	Image	Note
TS28: ovary secondary follicle	Present		8C	Expression was detected in granulosa cells.
TS28: theca of secondary follicle	Weak		8C

Structure	Level	Pattern	Image	Note
TS28: ovary primordial follicle	Present		8D	Squamous granulosa layer of a primordial follicle showed expression.

Structure	Level	Pattern	Image	Note
TS28: ovary antral follicle	Weak	Non-Uniform	8E	Granulosa cells of most antral follicles showed very weak to no expression.

Structure	Level	Pattern	Image	Note
TS28: corpus luteum	Strong	Regionally restricted	8F	Regressing corpora lutea contained cells that showed strong expression.

Structure	Level	Pattern	Image	Note
TS28: corpus luteum	Strong		9A , 9B	Variable levels of expression was noted between different cells that form the corpus luteum. Both large and small luteal cells showed expression.

Structure	Level	Pattern	Image	Note
TS22: ovary	Weak	Diffuse	10_E14.5' , 10_E14.5	Weak diffuse expression was detected in most cells forming the ovarian structure. Higher magnification images showed specific expression in the epithelial pregranulosa cells associated with the ovarian cysts.

Structure	Level	Pattern	Image	Note
TS26: ovary	Weak	Diffuse	10_E18.5' , 10_E18.5	Weak diffuse expression was detected in most cells forming the ovarian structure. Level of expression was higher at E18.5 than at E14.5. Higher magnification images showed specific expression in the epithelial pregranulosa cells associated with the ovarian cysts.

Structure	Level	Pattern	Image	Note
TS27: ovary	Strong	Regionally restricted	10_P0' , 10_P0	Expression at P0 was lower in the pregranulosa layer, but there was strong expression in the germinal epithelium.

Structure	Level	Pattern	Image	Note
TS28: mesenchymal stroma of ovary	Strong	Clusters	10_P7	Starting at P7, expression was observed in few clusters of interstitial cells.

Structure	Level	Pattern	Image	Note
TS28: mesenchymal stroma of ovary	Strong	Clusters	10_P14	Expression was observed in few clusters of interstitial cells that were very prominent.

Structure	Level	Pattern	Image	Note
TS28: mesenchymal stroma of ovary	Strong	Clusters	10_P21	Expression was observed in few clusters of interstitial cells that were very prominent. The expression pattern at P21 closely resembled that observed in the adult ovary.

Structure	Level	Pattern	Image	Note
TS28: oviduct	Strong		11A , 11A'

Structure	Level	Pattern	Image	Note
TS28: uterus epithelium	Strong		11B' , 11B	Expression was distributed throughout the cytoplasm with specific enrichment towards the apical regions of the cells.