Nefm Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:47640 Herbarth B, et al., Mutation of the Sry-related Sox10 gene in Dominant megacolon, a mouse model for human Hirschsprung disease. Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5161-5
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6842951
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Antibody:	anti-neurofilament clone 2H3
Detection system:	Secondary antibody

Results

3a Dom/Dom ©

Specimen 3a Dom/Dom: embryonic day 10.5; Sox10^Dom/Sox10^Dom (more

)

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present	Regionally restricted	3a Dom/Dom	Striking alterations in morphology of cranial nerves and ganglia.
TS17: trigeminal ganglion	Weak	Regionally restricted	3a Dom/Dom	Expression severely diminitished in dorsal portion of trigeminal ganglion.
TS17: glossopharyngeal ganglion	Present	Regionally restricted	3a Dom/Dom	Proximal portion of glossopharyngeal and vagus ganglia were considerably thinner than in heterozygous and wild-type mice.
TS17: vagus ganglion	Present	Regionally restricted	3a Dom/Dom	Proximal portion of glossopharyngeal and vagus ganglia were considerably thinner than in heterozygous and wild-type mice.
TS17: facial ganglion	Present	Regionally restricted	3a Dom/Dom	Morphological alterations in facial ganglion restricted to proximal portion of nerve root.

3a Dom/+ ©

Specimen 3a Dom/+: embryonic day 10.5; Sox10^Dom/Sox10⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: cranial ganglion	Present		3a Dom/+
TS17: cranial nerve	Present		3a Dom/+

Specimen 3c NF Dom/Dom: embryonic day 10.5; Sox10^Dom/Sox10^Dom (more

)

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		3c NF Dom/Dom	No defect in neurous of trunk neural crest. Expression in dorsal root ganglia and motor nerves appeared unaltered.
TS17: spinal nerve	Present		3c NF Dom/Dom	No defect in neurous of trunk neural crest. Expression in dorsal root ganglia and motor nerves appeared unaltered.

3c NF +/+ ©

Specimen 3c NF +/+: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		3c NF +/+
TS17: spinal nerve	Present		3c NF +/+

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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