Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:175228
Medeiros LA, et al., Mir-290-295 deficiency in mice results in partially penetrant embryonic lethality and germ cell defects. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14163-8
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6790208
|
|
Gene symbol:
|
Gcna
|
|
Gene name:
|
germ cell nuclear acidic protein
|
|
Antibody:
|
anti-GCNA1
|
|
Assay notes:
|
These data are descrobed in the text. Fixative was either 4% paraformaldehyde or Bouin's
solution.
|
|
|
Results
|
Specimen
female wt:
embryonic day 13.5
(more )
Specimen
female wt:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
Specimen
female -/-:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Mirc5tm1Jae/Mirc5tm1Jae
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: germ cell of ovary |
Present |
|
|
In this mutant there is a dramatic reduction in the number positive cells compared to control embryos.
|
Specimen
male wt:
embryonic day 13.5
(more )
Specimen
male wt:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
Specimen
male -/-:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Mirc5tm1Jae/Mirc5tm1Jae
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: germ cell of testis |
Present |
|
|
In this mutant there is a dramatic reduction in the number positive cells compared to control embryos.
|
|