Ddx4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:175228 Medeiros LA, et al., Mir-290-295 deficiency in mice results in partially penetrant embryonic lethality and germ cell defects. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14163-8
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6790207
Gene symbol:	Ddx4
Gene name:	DEAD box helicase 4

Antibody:	anti-MVH
Assay notes:	Fixative was either 4% paraformaldehyde or Bouin's solution.

Results

5A ©

Specimen 5A: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: germ cell of ovary	Present		5A

5B ©

Specimen 5B: embryonic day 13.5; Mirc5^tm1Jae/Mirc5^tm1Jae (more

)

Structure	Level	Pattern	Image	Note
TS21: germ cell of ovary	Present		5B	In this mutant there is a dramatic reduction in the number positive cells compared to control embryos.

5C ©

Specimen 5C: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		5C

5D ©

Specimen 5D: embryonic day 13.5; Mirc5^tm1Jae/Mirc5^tm1Jae (more

)

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		5D	In this mutant there is a dramatic reduction in the number positive cells compared to control embryos.

5F ©

Specimen 5F: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell of gonad	Present		5F

5G ©

Specimen 5G: embryonic day 11.5; Mirc5^tm1Jae/Mirc5^tm1Jae (more

)

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell of gonad	Present		5G	Most mutant genital ridges exhibited less than 5% as many germ cells as found in wild-type littermate controls.

5H ©

Specimen 5H: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell of gonad	Present		5H

5I ©

Specimen 5I: embryonic day 11.5; Mirc5^tm1Jae/Mirc5^tm1Jae (more

)

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell of gonad	Present		5I	Most mutant genital ridges exhibited less than 5% as many germ cells as found in wild-type littermate controls.

S1C ©

Specimen S1C: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		S1C

S1D ©

Specimen S1D: postnatal day 5; Mirc5^tm1Jae/Mirc5^tm1Jae (more

)

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		S1D	This mutant contained less than 20% as many oocytes as wild-type ovaries. The number of follicles and the primordial follicle pool are reduced in this mutant compared to wild-type.

S1G ©

Specimen S1G: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		S1G

S1H ©

Specimen S1H: postnatal day 10; Mirc5^tm1Jae/Mirc5^tm1Jae (more

)

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		S1H	The number of follicles and the primordial follicle pool are reduced in this mutant compared to wild-type.

S1K ©

Specimen S1K: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		S1K

S1L ©

Specimen S1L: postnatal day 5; Mirc5^tm1Jae/Mirc5^tm1Jae (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		S1L	The number of germ cells is reduced in this mutant compared to controls.

S1O ©

Specimen S1O: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		S1O

S1P ©

Specimen S1P: postnatal day 10; Mirc5^tm1Jae/Mirc5^tm1Jae (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		S1P	In this mutant germ cells continued to proliferate.

S4A ©

S4B ©

S4C ©

Specimen S4A: embryonic day 12.5 (more

)
Note: Double labeled: red - Ddx4; green - Pcna (assay MGI:6790234).

Structure	Level	Pattern	Image	Note
TS20: germ cell of ovary	Present		S4B , S4A , S4C

S4D ©

S4E ©

S4F ©

Specimen S4D: embryonic day 12.5; Mirc5^tm1Jae/Mirc5^tm1Jae (more

)
Note: Double labeled: red - Ddx4; green - Pcna (assay MGI:6790234).

Structure	Level	Pattern	Image	Note
TS20: germ cell of ovary	Present		S4E , S4F , S4D

S4G ©

S4H ©

S4I ©

Specimen S4G: embryonic day 12.5 (more

)
Note: Double labeled: red - Ddx4; green - Pcna (assay MGI:6790234).

Structure	Level	Pattern	Image	Note
TS20: germ cell of testis	Present		S4I , S4H , S4G

S4J ©

S4K ©

S4L ©

Specimen S4J: embryonic day 12.5; Mirc5^tm1Jae/Mirc5^tm1Jae (more

)
Note: Double labeled: red - Ddx4; green - Pcna (assay MGI:6790234).

Structure	Level	Pattern	Image	Note
TS20: germ cell of testis	Present		S4L , S4K , S4J

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