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Gene Expression Data
Assay Details
Assay
Reference: J:306246 He M, et al., Chloride channels regulate differentiation and barrier functions of the mammalian airway. Elife. 2020 Apr 14;9:e53085
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6781964
Gene symbol: Scgb1a1
Gene name: secretoglobin, family 1A, member 1
Antibody: anti-SCGB1A1 (B-6; sc-390313)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1 S1A wt: postnatal day 0 (more )
Note: Double labeled: magenta - Scgb1a1; green - Cdh1 (assay MGI:6781965).
Structure Level Pattern Image Note
TS27: trachea epithelium Present Regionally restricted 1 S1A upper left Expression is present in secretory cells.
Specimen 1 S1A Ano1-/-: postnatal day 0; Ano1tm1Bdh/Ano1tm1Bdh (more )
Note: Double labeled: magenta - Scgb1a1; green - Cdh1 (assay MGI:6781965).
Structure Level Pattern Image Note
TS27: trachea epithelium Present Regionally restricted 1 S1A upper right Expression is present in secretory cells; their number is massively expanded in the mutant.
Specimen 4C: postnatal day 3 (more )
Note: Double labeled: green - Scgb1a1; red - Foxj1 (assay MGI:6781970).
Structure Level Pattern Image Note
TS27: trachea epithelium Present Regionally restricted 4C MERGE , 4C SCGB1A1 Although this is considered a secretory cell marker, expression is also seen in "hybrid" cells that express ciliated cell markers.
Specimen 7G Proximal upper: postnatal day 25; Ano1tm2Jrr/Ano1+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: blue - Scgb1a1; magenta - centrin. Genotype assigned using figure label (not legend).
Structure Level Pattern Image Note
TS28: trachea epithelium Present Regionally restricted 7G ShhCre;Ano1fl/+ Proximal Expression is present in secretory cells.
Specimen 7G Distal upper: postnatal day 25; Ano1tm2Jrr/Ano1+, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: blue - Scgb1a1; magenta - centrin. Genotype assigned using figure label (not legend).
Structure Level Pattern Image Note
TS28: trachea epithelium Present Regionally restricted 7G ShhCre;Ano1fl/+ Distal Expression is present in secretory cells.
Specimen 7G Proximal lower: postnatal day 25; Ano1tm2Jrr/Ano1tm2Jrr, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: blue - Scgb1a1; magenta - centrin.
Structure Level Pattern Image Note
TS28: trachea epithelium Present Regionally restricted 7G ShhCre;Ano1fl/fl Proximal Expression is present in secretory cells; the number of these cells seen in the proximal trachea is comparable to those seen in wild type.
Specimen 7G Distal lower: postnatal day 25; Ano1tm2Jrr/Ano1tm2Jrr, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant. Double labeled: blue - Scgb1a1; magenta - centrin.
Structure Level Pattern Image Note
TS28: trachea epithelium Present Regionally restricted 7G ShhCre;Ano1fl/fl Distal Expression is present in secretory cells; the number of these cells seen in the distal trachea is greatly increased compared to those seen in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory