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Gene Expression Data
Assay Details
Assay
Reference: J:281028 Kiyama T, et al., Essential Roles of Tbr1 in the Formation and Maintenance of the Orientation-Selective J-RGCs and a Group of OFF-Sustained RGCs in Mouse. Cell Rep. 2019 Apr 16;27(3):900-915.e5
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6781280
Gene symbol: Tbr1
Gene name: T-box brain transcription factor 1
Reporter: GFP
Antibody: anti-GFP (#A10262)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 1G: postnatal day 30; Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+ (more )
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Single cells 1G Expression is seen in nasal, ventral and temporal regions of the retina.
Specimen 1H: postnatal day 30; Atoh7tm1Gan/Atoh7tm1Gan, Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+ (more )
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Trace 1H Only ~5% of retinal ganglion cells (RGC) are formed in this mutant; the lack of expression suggests the Tbr1+ cells are RGCs.
Specimen 1I: postnatal day 30; Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+ (more )
Note: Double labeled: red - Tbr1 reporter; green - Pou4f1 (assay MGI:6781817).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Single cells 1I Expression is seen in a subset of cells.
Specimen 1J: postnatal day 30; Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+ (more )
Note: Double labeled: red - Tbr1 reporter; blue - Pou4f2 (assay MGI:6781819).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Single cells 1J Expression is seen in a subset of cells.
Specimen S1A: postnatal day 30; Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+ (more )
Note: dorsal quadrant
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Single cells S1A This is the region with the least number of expressing cells (~3.7%).
Specimen S1B: postnatal day 30; Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+ (more )
Note: nasal quadrant
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Single cells S1B Expression is seen in ~5.5% of the cells in this region.
Specimen S1C: postnatal day 30; Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+ (more )
Note: ventral quadrant
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Single cells S1C Expression is seen in ~5.5% of the cells in this region.
Specimen S1D: postnatal day 30; Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+ (more )
Note: temporal quadrant
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Single cells S1D Expression is seen in ~5.5% of the cells in this region.
Specimen S2F: postnatal day 30; Tbr1tm1.1(cre/ERT2)Maoc/Tbr1+ (more )
Note: Triple labeled: red - Tbr1 reporter; blue - Pou4f2 (assay MGI:6781819); green - Nefh (assay MGI:6781950).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Single cells S2F Expression is seen in a subset of cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory