Nefm Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:309563 Rafipay A, et al., Knockdown of slit signaling during limb development leads to a reduction in humerus length. Dev Dyn. 2021 Sep;250(9):1340-1357
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6780210
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Antibody:	Anti-Neurofilament M (AB1987)
Detection system:	Secondary antibody coupled to Cy3

Results

3A ©

Specimen 3A: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb nerve	Present	Regionally restricted	3A	Expression in nerves entering forelimb.

3B ©

Specimen 3B: embryonic day 11.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb nerve	Present	Regionally restricted	3B	Expression not different between Slit1+/+, Slit1-/- and Slit1-/-Slit2-/- embryos.

3C ©

Specimen 3C: embryonic day 11.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl, Slit2^tm1Matl/Slit2^tm1Matl (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb nerve	Present	Regionally restricted	3C	Expression not different between Slit1+/+, Slit1-/- and Slit1-/-Slit2-/- embryos.

3A' ©

Specimen 3A': embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: limb nerve	Present	Regionally restricted	3A'	Expression in two main nerve branches entering hindlimb.

3B' ©

Specimen 3B': embryonic day 11.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl (more

)

Structure	Level	Pattern	Image	Note
TS19: limb nerve	Present	Regionally restricted	3B'	Expression not different between Slit1+/+, Slit1-/- and Slit1-/-Slit2-/- embryos.

3C' ©

Specimen 3C': embryonic day 11.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl, Slit2^tm1Matl/Slit2^tm1Matl (more

)

Structure	Level	Pattern	Image	Note
TS19: limb nerve	Present	Regionally restricted	3C'	Expression not different between Slit1+/+, Slit1-/- and Slit1-/-Slit2-/- embryos.

3D ©

Specimen 3D: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: forelimb nerve	Present	Regionally restricted	3D	Expression in nerves entering handplate.

3E ©

Specimen 3E: embryonic day 13.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl (more

)

Structure	Level	Pattern	Image	Note
TS21: forelimb nerve	Present	Regionally restricted	3E	No difference in pattern of expression between Slit1+/+, Slit1-/- and Slit1-/-Slit2-/- embryos.

3F ©

Specimen 3F: embryonic day 13.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl, Slit2^tm1Matl/Slit2^tm1Matl (more

)

Structure	Level	Pattern	Image	Note
TS21: forelimb nerve	Present	Regionally restricted	3F	No difference in pattern of expression between Slit1+/+, Slit1-/- and Slit1-/-Slit2-/- embryos.

3G ©

Specimen 3G: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: forelimb nerve	Present	Regionally restricted	3G	Expression in distal nerves, progressing to digit tips.

3H ©

Specimen 3H: embryonic day 14.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl (more

)

Structure	Level	Pattern	Image	Note
TS22: forelimb nerve	Present	Regionally restricted	3H	No difference in pattern of expression between Slit1+/+, Slit1-/- and Slit1-/-Slit2-/- embryos.

3I ©

Specimen 3I: embryonic day 14.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl, Slit2^tm1Matl/Slit2^tm1Matl (more

)

Structure	Level	Pattern	Image	Note
TS22: forelimb nerve	Present	Regionally restricted	3I	No difference in pattern of expression between Slit1+/+, Slit1-/- and Slit1-/-Slit2-/- embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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