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Gene Expression Data
Assay Details
Assay
Reference: J:310126 Quarta C, et al., Functional identity of hypothalamic melanocortin neurons depends on Tbx3. Nat Metab. 2019 Feb;1(2):222-235
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6766549
Gene symbol: Pomc
Gene name: pro-opiomelanocortin-alpha
Antibody: anti-Pomc (H-029-30)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 1c: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: magenta - Pomc; green - Tbx3 reporter (assay MGI:6764615).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 1c Pomc , 1c Merge Expression is seen in neurons.
Specimen 1d - E18.5: embryonic day 18.5; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: red - Pomc; green - Tbx3 reporter (assay MGI:6764615).
Structure Level Pattern Image Note
TS26: arcuate nucleus Present Regionally restricted 1d E18.5 Expression is seen in neurons.
Specimen 1d - P0: postnatal day 0; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: red - Pomc; green - Tbx3 reporter (assay MGI:6764615).
Structure Level Pattern Image Note
TS27: arcuate nucleus Present Regionally restricted 1d P0 Expression is seen in neurons.
Specimen 1d - P4: postnatal day 4; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: red - Pomc; green - Tbx3 reporter (assay MGI:6764615).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 1d P4 Expression is seen in neurons.
Specimen 1d - Adult: postnatal adult; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: red - Pomc; green - Tbx3 reporter (assay MGI:6764615).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 1d Adult Expression is seen in neurons.
Specimen 4a - control: postnatal day 4; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Tg(Pomc1-cre)16Lowl/0 (more )
Note: Conditional mutant. Double labeled: magenta - Pomc; green - Cre reporter.
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 4a Pomc upper , 4a Merge upper Expression is seen in neurons.
Specimen 4a - KO: postnatal day 4; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Tbx3tm3.1Moon/Tbx3tm3.1Moon, Tg(Pomc1-cre)16Lowl/0 (more )
Note: Conditional mutant. Double labeled: magenta - Pomc; green - Cre reporter.
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 4a Merge lower , 4a Pomc lower Expression is seen in neurons; there are significantly fewer expressing neurons in the mutant compared to wild type.
Specimen 4d - control: postnatal adult; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Tg(Pomc1-cre)16Lowl/0 (more )
Note: Conditional mutant. Double labeled: magenta - Pomc; green - Cre reporter.
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 4d Pomc upper , 4d Merge upper Expression is seen in neurons.
Specimen 4d - KO: postnatal adult; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Tbx3tm3.1Moon/Tbx3tm3.1Moon, Tg(Pomc1-cre)16Lowl/0 (more )
Note: Conditional mutant. Double labeled: magenta - Pomc; green - Cre reporter.
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 4d Pomc lower , 4d Merge lower Expression is seen in neurons; there are significantly fewer expressing neurons in the mutant compared to wild type.
Specimen 4g - control: postnatal adult; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Tg(Pomc1-cre)16Lowl/0 (more )
Note: Conditional mutant. Double labeled: magenta - Pomc; green - Cre reporter.
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present Regionally restricted 4g Merge upper , 4g Pomc upper Expression is seen in neurons.
Specimen 4g - KO: postnatal adult; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Tbx3tm3.1Moon/Tbx3tm3.1Moon, Tg(Pomc1-cre)16Lowl/0 (more )
Note: Conditional mutant. Double labeled: magenta - Pomc; green - Cre reporter.
Structure Level Pattern Image Note
TS28: paraventricular hypothalamic nucleus Present Regionally restricted 4g Merge lower , 4g Pomc lower Expression is seen in neurons; expression in the mutant is reduced compared to that seen in wild type.
Specimen S1m: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: magenta - Pomc; green - Tbx3 reporter (assay MGI:6764615).
Structure Level Pattern Image Note
TS28: solitary tract nucleus Present Regionally restricted S1m Merge , S1m Pomc Expression is seen in neurons.
Specimen S1n: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: magenta - Pomc; green - Tbx3 reporter (assay MGI:6764615).
Structure Level Pattern Image Note
TS28: neurohypophysis Present Regionally restricted S1n Merge , S1n Pomc Expression is seen in neurons.
Specimen S5c - control: postnatal week 8; Tbx3tm3.1Moon/Tbx3tm3.1Moon (more )
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted S5c Ad libitum upper Expression is seen in neurons.
Specimen S5c - KO: postnatal week 8; Tbx3tm3.1Moon/Tbx3tm3.1Moon, Tg(Pomc1-cre)16Lowl/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted S5c Ad libitum lower Expression is seen in neurons; their number is significantly decreased in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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