Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80021
Pringle NP, et al., Fgfr3 expression by astrocytes and their precursors: evidence that astrocytes and oligodendrocytes originate in distinct neuroepithelial domains. Development. 2003 Jan;130(1):93-102
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6765506
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Gene symbol:
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Fgfr3
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Gene name:
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fibroblast growth factor receptor 3
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Probe:
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Fgfr3 probe18
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Probe preparation:
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labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1F:
embryonic day 13.5
(more  )
Specimen
1F:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord cervical region |
Present |
Regionally restricted |
1F
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Authors state a progression of expression occurs in mouse similar to that seen in chick embryos.
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Specimen
1G:
embryonic day 14.5
(more )
Specimen
1G:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord cervical region |
Present |
Regionally restricted |
1G
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Two spatially separated domains of expression. Ventral 'gap' is not so pronounced as in chick embryos.
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Specimen
3A/C:
embryonic day 18.0
(more )
Note: Double labeled: blue - Fgfr3; green - BrdU.
Specimen
3A/C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Fgfr3; green - BrdU.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
Regionally restricted |
3A
,
3C
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Expression in cells that have incorporated BrdU as well as cells that have not incorporate BrdU.
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Specimen
5A:
postnatal newborn
(more )
Specimen
5A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pdgfatm1Cbet/Pdgfatm1Cbet
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: spinal cord |
Present |
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5B
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Expression unaffected compared with wild-type. Neither number of distribution of expressing cells is noticeably changed.
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Specimen
6B:
embryonic day 18.0
(more )
Specimen
6B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord cervical region |
Present |
Regionally restricted |
6B
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Expression in Gfap-expressing astrocytes. Majority of expressing cells in grey matter cells are Gfap-negative.
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Analysis Tools