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Gene Expression Data
Assay Details
Assay
Reference: J:310126 Quarta C, et al., Functional identity of hypothalamic melanocortin neurons depends on Tbx3. Nat Metab. 2019 Feb;1(2):222-235
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6764615
Gene symbol: Tbx3
Gene name: T-box 3
Reporter: Venus
Antibody: anti-GFP (ab6673)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1a: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 1a Expression is seen in neurons.
Specimen 1c: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; magenta - Pomc (assay MGI:6766549).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 1c Venus , 1c Merge Expression is seen in neurons.
Specimen 1d - E18.5: embryonic day 18.5; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pomc (assay MGI:6766549).
Structure Level Pattern Image Note
TS26: arcuate nucleus Present Regionally restricted 1d E18.5 Expression is seen in neurons.
Specimen 1d - P0: postnatal day 0; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pomc (assay MGI:6766549).
Structure Level Pattern Image Note
TS27: arcuate nucleus Present Regionally restricted 1d P0 Expression is seen in neurons.
Specimen 1d - P4: postnatal day 4; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pomc (assay MGI:6766549).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 1d P4 Expression is seen in neurons.
Specimen 1d - Adult: postnatal adult; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Pomc (assay MGI:6766549).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted 1d Adult Expression is seen in neurons.
Specimen S1b: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Structure Level Pattern Image Note
TS28: solitary tract nucleus Present Regionally restricted S1b Expression is seen in neurons.
Specimen S1f - ARC: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Tbx3 protein (assay MGI:6766567).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted S1f ARC Expression is seen in neurons.
Specimen S1f - NTS: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; red - Tbx3 protein (assay MGI:6766567).
Structure Level Pattern Image Note
TS28: solitary tract nucleus Present Regionally restricted S1f NTS Expression is seen in neurons.
Specimen S1m: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; magenta - Pomc (assay MGI:6766549).
Structure Level Pattern Image Note
TS28: solitary tract nucleus Present Regionally restricted S1m Merge , S1m Venus Expression is seen in neurons.
Specimen S1n: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; magenta - Pomc (assay MGI:6766549).
Structure Level Pattern Image Note
TS28: neurohypophysis Present S1n Venus , S1n Merge Expression is seen in neurons.
TS28: adenohypophysis Absent S1n Venus , S1n Merge
Specimen S1o: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; magenta - Gfap (assay MGI:6766568).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted S1o Merge , S1o Venus Expression is not seen in astrocytes.
Specimen S1p: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; magenta - Aif1 (assay MGI:6766569).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted S1p Venus , S1p Merge Expression is not seen in microglia.
Specimen S1q: postnatal week 12; Tbx3em1(cre)Mhts/Tbx3+ (more )
Note: Double labeled: green - Tbx3 reporter; magenta - Vim (assay MGI:6766570).
Structure Level Pattern Image Note
TS28: arcuate nucleus Present Regionally restricted S1q Merge , S1q Venus Expression is seen in tanycytes/reactive astrocytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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