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Gene Expression Data
Assay Details
Assay
Reference: J:115783 Kiernan AE, et al., The Notch ligand JAG1 is required for sensory progenitor development in the mammalian inner ear. PLoS Genet. 2006 Jan;2(1):e4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6763035
Gene symbol: Sox2
Gene name: SRY (sex determining region Y)-box 2
Antibody: anti-Sox2 (AB5603)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 9B: embryonic day 12.5 (more )
Note: basal region
Structure Level Pattern Image Note
TS20: cochlear duct epithelium Present Regionally restricted 9B Expression did overlap with the Jag1 in the prosensory domains in the basal regions.
Specimen 9C: embryonic day 12.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. basal region
Structure Level Pattern Image Note
TS20: cochlear duct epithelium Weak Regionally restricted 9C Expression was absent from the basal regions and significantly downregulated in the apical regions.
Specimen 9E: embryonic day 12.5 (more )
Note: apical region
Structure Level Pattern Image Note
TS20: cochlear duct epithelium Present Regionally restricted 9E In the apical regions, where the sensory precursors have ceased dividing, expression of Jag1 and Sox2 did not overlap.
Specimen 9F: embryonic day 12.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. apical region
Structure Level Pattern Image Note
TS20: cochlear duct epithelium Weak Regionally restricted 9F Significantly downregulated expression in the apical regions.
Specimen 10B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: semicircular canal Present Regionally restricted 10B Jag1 and Sox2 exhibited largely overlapping expression in posterior cristae and saccular macula. In the posterior cristae, expression had a negative patch in the middle, whereas Sox2 expression did not.
Specimen 10C: embryonic day 12.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: semicircular canal Present Regionally restricted 10C Displayed fairly normal expression in saccular macula. The posterior cristae was missing in the mutant.
Specimen 10E: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: utricle epithelium Present Regionally restricted 10E Jag1 and Sox2 exhibited largely overlapping expression domains.
Specimen 10F: embryonic day 12.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: utricle epithelium Trace Regionally restricted 10F Very weak expression and the expression domain was much smaller than in controls.
Specimen 10H: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: semicircular canal Present Regionally restricted 10H Jag1 and Sox2 exhibited largely overlapping expression in anterior and lateral cristae. In the anterior cristae, expression had a negative patch in the middle, whereas Sox2 expression did not.
Specimen 10I: embryonic day 12.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: semicircular canal Trace Regionally restricted 10I The entire ampullae appeared to be missing or severely disrupted in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory