Antibody:	anti-Jag1 (H-114; sc-8303)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS20: blood vessel endothelium	Present		9A
TS20: cochlear duct epithelium	Present	Regionally restricted	9A	Expression did overlap with the Sox2+ prosensory domains in the basal regions.

Structure	Level	Pattern	Image	Note
TS20: blood vessel endothelium	Present		9D
TS20: cochlear duct epithelium	Present	Regionally restricted	9D	In the apical regions, where the sensory precursors have ceased dividing, expression of Jag1 and Sox2 did not overlap.

Structure	Level	Pattern	Image	Note
TS20: semicircular canal	Present	Regionally restricted	10A	Jag1 and Sox2 exhibited largely overlapping expression in posterior cristae and saccular macula. In the posterior cristae, expression had a negative patch in the middle, whereas Sox2 expression did not.

Structure	Level	Pattern	Image	Note
TS20: utricle epithelium	Present	Regionally restricted	10D	Jag1 and Sox2 exhibited largely overlapping expression domains.

Structure	Level	Pattern	Image	Note
TS20: semicircular canal	Present	Regionally restricted	10G	Jag1 and Sox2 exhibited largely overlapping expression in anterior and lateral cristae. In the anterior cristae, expression had a negative patch in the middle, whereas Sox2 expression did not.