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Gene Expression Data
Assay Details
Assay
Reference: J:297051 Wang S, et al., RYK-mediated filopodial pathfinding facilitates midgut elongation. Development. 2020 Oct 27;147(20):dev195388
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6761395
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-cleaved Caspase-3 (Asp175; #9661)
Detection system: Secondary antibody
Assay notes: The antibody used recognizes the cleaved form of the protein.
Results
Specimen 7A: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: midgut epithelium Present Single cells 7A , 7A' Expression is in an occasional cell (shown is the absence of apoptotic fragments.)
Specimen 7B: embryonic day 14.5; Ryktm1Stac/Ryktm1Stac (more )
Structure Level Pattern Image Note
TS22: midgut epithelium Present Single cells 7B , 7B' Expression is at the apical side of the epithelium.
Specimen 7C: embryonic day 14.5; Ryktm1Stac/Ryktm1Stac (more )
Structure Level Pattern Image Note
TS22: midgut epithelium Present Single cells 7C' , 7C Expression is at the apical side of the epithelium.
Specimen 7D E12.5 Ctrl: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midgut epithelium Present Single cells 7D E12.5 control Apoptotic fragments were seen on approximately 0.5% of the cells.
Specimen 7D E12.5 Ryk-/-: embryonic day 12.5; Ryktm1Stac/Ryktm1Stac (more )
Structure Level Pattern Image Note
TS20: midgut epithelium Present Single cells 7D E12.5 Ryk-/- The number of apoptotic fragments increased to approximately 4% of the cells in this mutant compared to controls.
Specimen 7D E14.5 Ctrl: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: midgut epithelium Present Single cells 7D E14.5 control Apoptotic fragments were seen on approximately 0.5% of the cells.
Specimen 7D E14.5 Ryk-/-: embryonic day 14.5; Ryktm1Stac/Ryktm1Stac (more )
Structure Level Pattern Image Note
TS22: midgut epithelium Present Single cells 7D E14.5 Ryk-/- The number of apoptotic fragments increased to approximately 4% of the cells in this mutant compared to controls.
Specimen S1D Ror2fl/fl;RosamTmG/+: embryonic day 14.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Ror2tm1.1Meg/Ror2tm1.1Meg (more )
Structure Level Pattern Image Note
TS22: midgut Absent S1D Ror2fl/fl;RosamTmG/+ bottom , S1D Ror2fl/fl;RosamTmG/+ top
Specimen S1D ShhCre/+;Ror2fl/fl;RosamTmG/+: embryonic day 14.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Ror2tm1.1Meg/Ror2tm1.1Meg, Shhtm1(EGFP/cre)Cjt/Shh+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: midgut Absent S1D ShhCre/+;Ror2fl/fl;RosamTmG/+ top , S1D ShhCre/+;Ror2fl/fl;RosamTmG/+ bottom
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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