Antibody:	ADK antibody (A304-280A)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	3J (right) , 3J (left)	Co-expression with Gfap in astrocytes in cerebrum

Structure	Level	Pattern	Image	Note
TS27: cerebellar cortex	Present	Widespread	5C , 5A	Widespread co-expression with Ki67 in entire developing cerebellar cortex.
TS27: cerebellum external granule cell layer	Strong	Regionally restricted	5A , 5C	Robust expression at outermost external granular layer.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		5D , 5F
TS27: cerebellum external granule cell layer	Present		5D , 5F

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		5D inset
TS27: cerebellum external granule cell layer	Present		5D inset

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5G , 5I
TS28: cerebellum external granule cell layer	Present		5G , 5I
TS28: cerebellum internal granule cell layer	Present		5G , 5I
TS28: cerebellum white matter	Present		5I , 5G

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong	Regionally restricted	5G inset	Co-expression with Ki67. Expression initially increases in external granular layer. Number of double-expressing cells significantly increased compared with P0.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Weak	Regionally restricted	5J , 5L	Co-expression with Ki67. Number of double-expressing cells significantly reduced compared with P0, P2 and P5.
TS28: cerebellum external granule cell layer	Weak		5L , 5J	Expression decreases in external granular layer.
TS28: cerebellum white matter	Weak		5J , 5L	Expression in white matter region declined at P9.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Absent			No expression in white matter at P21.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Punctate	6A P5	Co-expression with Calb1 in Purkinje layer. Nuclear expression in most Purkinje cells.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong	Punctate	6A P5 inset	Prominent punctate nuclear expression in Purkinje cells at P5.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong	Punctate	6A P9	Co-expression with Calb1 in Purkinje layer. Prominent punctate nuclear expression in most Purkinje cells at P5.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	6A P21	Co-expression with Calb1 in Purkinje layer. Expression significantly reduced compared with P5.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	6C P21 (right)	Expression in Purkinje cells less prominent and only maintained in a few cells. Expression in clusters of cells between Purkinje cells in Purkinje layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Present	Regionally restricted	6D P5	Co-expression with Gfap in astrocytes.

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Present	Regionally restricted	6D P21	Co-expression with Gfap in astrocytes.

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Present	Regionally restricted	6D Adult	Co-expression with Gfap in astrocytes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Punctate	6E P5 (left)	Punctate nuclear expression in Bergmann glial cells in Purkinje layer. Expression in cell nuclei surrounded by Gfap-expressing cell somas.
TS28: cerebellum white matter	Present	Regionally restricted	6E P5 (left)	Co-expression with Gfap in astrocytes. Prominent expression in white matter.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Punctate	6E P21 (left)	Punctate nuclear expression in Bergmann glial cells in Purkinje layer. Expression in cell nuclei surrounded by Gfap-expressing cell somas.
TS28: cerebellum internal granule cell layer	Strong	Regionally restricted	6E P21 (left)	Co-expression with Gfap in astrocytes. Strong expression in glial cells in white matter and inner granular layer.
TS28: cerebellum white matter	Strong	Regionally restricted	6E P21 (left)	Co-expression with Gfap in astrocytes. Strong expression in glial cells in white matter and inner granular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Punctate	6E P21 (right)	Punctate nuclear expression in Bergmann glial cells in Purkinje layer. Expression in cell nuclei surrounded by Gfap-expressing cell somas.
TS28: cerebellum internal granule cell layer	Strong	Regionally restricted	6E P21 (right)	Co-expression with Gfap in astrocytes. Strong expression in glial cells in white matter and inner granular layer.
TS28: cerebellum white matter	Strong	Regionally restricted	6E P21 (right)	Co-expression with Gfap in astrocytes. Strong expression in glial cells in white matter and inner granular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Punctate	6E Adult (left)	Punctate nuclear expression in Bergmann glial cells in Purkinje layer. Expression in cell nuclei surrounded by Gfap-expressing cell somas.
TS28: cerebellum internal granule cell layer	Strong	Regionally restricted	6E Adult (left)	Co-expression with Gfap in astrocytes. Strong expression in glial cells in white matter and inner granular layer.
TS28: cerebellum white matter	Strong	Regionally restricted	6E Adult (left)	Co-expression with Gfap in astrocytes. Strong expression in glial cells in white matter and inner granular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Punctate	6E Adult (right)	Punctate nuclear expression in Bergmann glial cells in Purkinje layer.
TS28: cerebellum internal granule cell layer	Strong	Regionally restricted	6E Adult (right)	Co-expression with Gfap in astrocytes. Strong expression in glial cells in white matter and inner granular layer.
TS28: cerebellum white matter	Strong	Regionally restricted	6E Adult (right)	Co-expression with Gfap in astrocytes. Strong expression in glial cells in white matter and inner granular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Present		6F P5 (middle) , 6F P5 (right)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	6F P21 (right) , 6F P21 (middle left)	Expression in Purkinje layer. Expression in Bergman glial cells between Purkinje neurons.

Structure	Level	Pattern	Image	Note
TS27: cortical layer III	Present	Regionally restricted	7A P0	Nuclear co-localization of expression with NeuN (Rbfox3) in cortical neurons in layers III and IV of neocortex.
TS27: cortical layer IV	Present	Regionally restricted	7A P0	Nuclear co-localization of expression with NeuN (Rbfox3) in cortical neurons in layers III and IV of neocortex.

Structure	Level	Pattern	Image	Note
TS27: cortical layer III	Present	Regionally restricted	7A P2	Nuclear co-localization of expression with NeuN (Rbfox3) in cortical neurons in layers III and IV of neocortex.
TS27: cortical layer IV	Present	Regionally restricted	7A P2	Nuclear co-localization of expression with NeuN (Rbfox3) in cortical neurons in layers III and IV of neocortex.

Structure	Level	Pattern	Image	Note
TS28: cortical layer III	Present	Regionally restricted	7A P5	Nuclear co-localization of expression with NeuN (Rbfox3) in cortical neurons in layers III and IV of neocortex.
TS28: cortical layer IV	Present	Regionally restricted	7A P5	Nuclear co-localization of expression with NeuN (Rbfox3) in cortical neurons in layers III and IV of neocortex.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	7A Adult	No co-expression with NeuN (Rbfox3). Expression in NeuN-negative cells.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	7B P2	Nuclear co-localization of expression with NeuN (Rbfox3) in cortical neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	7B P5 ADK , 7B P5 NeuN/ADK	Nuclear co-localization of expression with NeuN (Rbfox3) in cortical neurons.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present	Regionally restricted	7C P0	Co-expression with NeuN (Rbfox3) in cerebellar neurons. Expression in innermost layer of external granular layer.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present	Regionally restricted	7C P0 inset	Expression in innermost layer of external granular layer.
TS27: cerebellum Purkinje cell layer	Present		7C P0 inset

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Present	Regionally restricted	7C P2	Co-expression with NeuN (Rbfox3) in cerebellar neurons. Expression in external granular layer.
TS27: cerebellum Purkinje cell layer	Strong	Regionally restricted	7C P2	Strong expression in NeuN (Rbfox3)-negative cells in Purkinje layer.
TS27: cerebellum molecular layer	Present	Regionally restricted	7C P2	Co-expression with NeuN (Rbfox3) in neurons in molecular layer.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Strong	Regionally restricted	7C P2 inset , 7D (upper)	Strong expression in NeuN (Rbfox3)-negative cells in Purkinje layer.
TS27: cerebellum molecular layer	Present	Regionally restricted	7D (upper) , 7C P2 inset	Co-expression with NeuN (Rbfox3) in neurons in molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	7E P5	Co-expression with NeuN (Rbfox3) in cerebellar neurons. Expression in external granular layer and Purkinje layer.
TS28: cerebellum external granule cell layer	Present	Regionally restricted	7E P5	Co-expression with NeuN (Rbfox3) in cerebellar neurons. Expression in external granular layer and Purkinje layer.
TS28: cerebellum white matter	Present		7E P5

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present	Regionally restricted	7E P5 inset	Co-expression with NeuN (Rbfox3) in cerebellar neurons. Expression in external granular layer and Purkinje layer.
TS28: cerebellum white matter	Present		7E P5 inset

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present	Regionally restricted	7E Adult	Co-expression with NeuN (Rbfox3) restricted to mature granule neurons in internal granular layer. Only a subset of cells express intense nuclear staining; majority of cells exhibit more diffuse, lower intensity expression.
TS28: cerebellum molecular layer	Present	Diffuse	7E Adult	Diffuse cytoplasmic staining in their parallel fibers in the molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present	Regionally restricted	7E Adult inset , 7F (upper)	Co-expression with NeuN (Rbfox3) restricted to mature granule neurons in internal granular layer. Only a subset of cells express intense nuclear staining; majority of cells exhibit more diffuse, lower intensity expression.
TS28: cerebellum molecular layer	Present	Diffuse	7E Adult inset , 7F (upper)	Diffuse cytoplasmic staining in their parallel fibers in the molecular layer.