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Gene Expression Data
Assay Details
Assay
Reference: J:306155 Goodyer WR, et al., Transcriptomic Profiling of the Developing Cardiac Conduction System at Single-Cell Resolution. Circ Res. 2019 Aug 2;125(4):379-397
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6730294
Gene symbol: Igfbp5
Gene name: insulin-like growth factor binding protein 5
Antibody: IGFBP-5 Antibody (AF578)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 2F: postnatal day 4 (more )
Note: Double labeled: red - Igfbp5; green - Hcn4 (assay MGI:6730292).
Structure Level Pattern Image Note
TS28: sinoatrial node Present 2F3 , 2F1 , 2F4 Expressed within the compact sinoatrial node, but not in the surrounding atrial working myocardium.
Specimen 8B-0: embryonic day 16.5 (more )
Note: optically cleared heart using iDISCO+
Structure Level Pattern Image Note
TS24: impulse conducting system Present Widespread 8B1 Expressed within the entire system, including sinoatrial node, atrioventricular node, His, bundle brunches, and purkinje fiber network.
TS24: atrioventricular bundle Present 8B1
TS24: sinoatrial node Present 8B1
Specimen 8B-90: embryonic day 16.5 (more )
Note: optically cleared heart using iDISCO+
Structure Level Pattern Image Note
TS24: impulse conducting system Present Widespread 8B2 Expressed within the entire system, including sinoatrial node, atrioventricular node, His, bundle brunches, and purkinje fiber network.
TS24: atrioventricular bundle Present 8B2
TS24: sinoatrial node Present 8B2
Specimen 8B-180: embryonic day 16.5 (more )
Note: optically cleared heart using iDISCO+
Structure Level Pattern Image Note
TS24: impulse conducting system Present Widespread 8B3 Expressed within the entire system, including sinoatrial node, atrioventricular node, His, bundle brunches, and purkinje fiber network.
TS24: atrioventricular bundle Present 8B3
TS24: sinoatrial node Present 8B3
Specimen 8B-270: embryonic day 16.5 (more )
Note: optically cleared heart using iDISCO+
Structure Level Pattern Image Note
TS24: impulse conducting system Present Widespread 8B4 Expressed within the entire system, including sinoatrial node, atrioventricular node, His, bundle brunches, and purkinje fiber network.
TS24: atrioventricular bundle Present 8B4
TS24: sinoatrial node Present 8B4
Specimen S4: postnatal day 12 (more )
Note: Double labeled: red - Igfbp5; green - Gja5 (assay MGI:6730299).
Structure Level Pattern Image Note
TS28: sinoatrial node Present S4_merge , S4_Igfbp5 Expressed within the compact sinoatrial node, but not in the sinoatrial node artery.
Specimen S5A: embryonic day 16.5 (more )
Note: Triple labeled: red - Igfbp5; green - Gja5 (assay MGI:6730299); blue - Hcn4 (assay MGI:6730359).
Structure Level Pattern Image Note
TS24: sinoatrial node Present S5A1 , S5A6 , S5A2 , S5A5 Expressed in the compact sinoatrial node and transitional cells.
Specimen S8A1: postnatal day 4 (more )
Note: Double labeled: red - Igfbp5; green - Gja5 (assay MGI:6730299).
Structure Level Pattern Image Note
TS28: atrioventricular node Present S8A1
TS28: impulse conducting system Present Regionally restricted S8A1 Expressed in the internodal tracks.
Specimen S8A2: postnatal day 4 (more )
Note: Double labeled: red - Igfbp5; green - Gja5 (assay MGI:6730299).
Structure Level Pattern Image Note
TS28: atrioventricular node Present S8A2
TS28: right branch of atrioventricular bundle Present S8A2
TS28: impulse conducting system Present Regionally restricted S8A2 Expressed in the internodal tracks.
Specimen S8A3: postnatal day 4 (more )
Note: Double labeled: red - Igfbp5; green - Gja5 (assay MGI:6730299).
Structure Level Pattern Image Note
TS28: left branch of atrioventricular bundle Present S8A3
TS28: right branch of atrioventricular bundle Present S8A3
TS28: impulse conducting system Present Regionally restricted S8A3 Expressed in the His bundle and the internodal tracks.
Specimen S8A4: postnatal day 4 (more )
Note: Double labeled: red - Igfbp5; green - Gja5 (assay MGI:6730299).
Structure Level Pattern Image Note
TS28: atrioventricular node Present S8A4
TS28: right branch of atrioventricular bundle Present S8A4
TS28: impulse conducting system Present Regionally restricted S8A4 Expressed in the His bundle.
Specimen S10A: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: impulse conducting system Present S10A Expressed in the His bundle, left and right bundle branches, and purkinje fibers.
Specimen S11B1: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: atrioventricular bundle Present S11B1
TS24: sinoatrial node Present S11B1
TS24: impulse conducting system Present S11B1 Expressed in the atrioventricular node and purkinje fibers.
Specimen S11B2: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: impulse conducting system Present Regionally restricted S11B2 Expressed in the His bundle, purkinje fibers and left and right bundle branches.
Specimen S11C-0: embryonic day 16.5 (more )
Note: Double labeled: red - Igfbp5; green - Gja5 (assay MGI:6730299).
Structure Level Pattern Image Note
TS24: sinoatrial node Present S11C1
TS24: atrium cardiac muscle Absent S11C1
Specimen S11C-90: embryonic day 16.5 (more )
Note: Double labeled: red - Igfbp5; green - Gja5 (assay MGI:6730299).
Structure Level Pattern Image Note
TS24: sinoatrial node Present S11C2
TS24: atrium cardiac muscle Absent S11C2
Specimen S11C-180: embryonic day 16.5 (more )
Note: Double labeled: red - Igfbp5; green - Gja5 (assay MGI:6730299).
Structure Level Pattern Image Note
TS24: sinoatrial node Present S11C3
TS24: atrium cardiac muscle Absent S11C3
Specimen S11C-270: embryonic day 16.5 (more )
Note: Double labeled: red - Igfbp5; green - Gja5 (assay MGI:6730299).
Structure Level Pattern Image Note
TS24: sinoatrial node Present S11C4
TS24: atrium cardiac muscle Absent S11C4
Specimen S12B-0: postnatal day 0 (more )
Note: optically cleared heart using iDISCO+
Structure Level Pattern Image Note
TS27: sinoatrial node Present S12B1
TS27: impulse conducting system Present Regionally restricted S12B1 Expressed in transitional sinoatrial conduction pathways.
Specimen S12B-90: postnatal day 0 (more )
Note: optically cleared heart using iDISCO+
Structure Level Pattern Image Note
TS27: sinoatrial node Present S12B2
TS27: impulse conducting system Present Regionally restricted S12B2 Expressed in transitional sinoatrial conduction pathways.
Specimen S12B-225: postnatal day 0 (more )
Note: optically cleared heart using iDISCO+
Structure Level Pattern Image Note
TS27: sinoatrial node Present S12B3
TS27: impulse conducting system Present Regionally restricted S12B3 Expressed in transitional sinoatrial conduction pathways.
Specimen not shown: postnatal (more )
Structure Level Pattern Image Note
TS28: endocardial lining Absent Could not be detected.
TS28: visceral pericardium Absent Could not be detected.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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