Probe:	Cyp21a1 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present	Regionally restricted	1A	Expression mainly in distal epithelium during pseudoglandular stage.
TS23: lung mesenchyme	Absent		1A

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Present	Regionally restricted	1C	Expression mainly in distal epithelium during canalicular stage. Expression in some proximal epithelia; no espression in others.
TS25: lung mesenchyme	Absent		1C

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Present	Regionally restricted	1D	Expression mainly in distal epithelium during canalicular stage. Expression in developing distal epithelium with no lumina.
TS25: lung mesenchyme	Absent		1D

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Present	Regionally restricted	1F	Expression mainly in distal epithelium during canalicular stage.
TS25: lung mesenchyme	Absent		1F

Structure	Level	Pattern	Image	Note
TS25: lung vascular element	Present	Regionally restricted	1H	No expression in endothelial cells in veins.

Structure	Level	Pattern	Image	Note
TS25: lung vascular element	Present	Regionally restricted	1I	Expression in tunica adventitia surrounding arteries. No expression in arterial endothelial cells and smooth muscle surrounding arteries.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Strong	Regionally restricted	2A	Strong expression in proixmal epithelium. Distal epithelium generally negative for expression in regions with high cellularity in mesenchymal compartment.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Strong	Regionally restricted	2C	Strong expression in proixmal epithelium. Distal epithelium generally negative for expression in regions with high cellularity in mesenchymal compartment.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present	Regionally restricted	2E	Expression in distal epithelium in regions with large lumina where saccules are developing.

Structure	Level	Pattern	Image	Note
TS26: lung vascular element	Present	Regionally restricted	2G	Expression absent in endothelial cells of some veins.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Strong	Regionally restricted	2H	Strong expression in proxmial epithelium. Distal epithelium generally negative for expression in regions with high cellularity in mesenchymal compartment.
TS26: lung vascular element	Present	Regionally restricted	2H	Expression present in endothelial cells surrounding some veins.

Structure	Level	Pattern	Image	Note
TS26: lung vascular element	Present	Regionally restricted		Expression in tunica adventitia surrounding arteries. No expression in arterial endothelial cells and smooth muscle surrounding arteries.

Structure	Level	Pattern	Image	Note
TS27: lung epithelium	Present	Regionally restricted	2J	Expression in distal epithelium with thin walls in regions of developing saccules.

Structure	Level	Pattern	Image	Note
TS27: lung epithelium	Present	Regionally restricted	2L	Expression in proximal epithelium.

Structure	Level	Pattern	Image	Note
TS27: lung vascular element	Present	Regionally restricted		Expression in tunica adventitia surrounding arteries. No expression in arterial endothelial cells and smooth muscle surrounding arteries.

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	3A	Expression in very thin walls in respiratory region. No expression in proximal epithelium.

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	3C	Expression in very thin walls in respiratory region. No expression in proximal epithelium.

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	3D	Expression in walls of distal epithelium. No expression in proximal epithelium.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present	Regionally restricted	3F	Expression in tunica adventitia of arteries. No expression in endothelial cells of arteries.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present	Regionally restricted	3H	No expression in endothelial cells of veins.

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	4A	Expression mainly associated with wall of respiratory zone. Expression in some but not all septa

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	4C	Expression mainly associated with wall of respiratory zone. Expression in some but not all septa

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	5E	Expression in some but not all septa.