Gene Expression Data
Assay Details
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Assay
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Reference:
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J:278517
Li M, et al., The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. Sci Adv. 2019;5(8):eaax1101
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6727344
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Gene symbol:
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Ddx4
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Gene name:
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DEAD box helicase 4
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Results
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Specimen
6A:
embryonic day 13.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6B:
(close )
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Genetic Background:
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C57BL/6-Zcwpw1em1Kliu
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Mutant Allele(s):
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Zcwpw1em1Kliu/Zcwpw1em1Kliu
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: germ cell of ovary |
Present |
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6B
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Number of female germ cells comparable to wild type ovaries when meiosis is about to start or just starting.
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Specimen
6D:
postnatal day 1
(more )
Specimen
6D:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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postnatal day 1
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6E:
(close )
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Genetic Background:
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C57BL/6-Zcwpw1em1Kliu
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Mutant Allele(s):
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Zcwpw1em1Kliu/Zcwpw1em1Kliu
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Age:
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postnatal day 1
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: oocyte |
Present |
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6E
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Expression in only 55% of oocytes, largely reduced compared to wild type ovaries, when meiosis prophase I was about to be finished.
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Specimen
6G:
postnatal day 8
(more )
Specimen
6G:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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postnatal day 8
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6H:
(close )
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Genetic Background:
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C57BL/6-Zcwpw1em1Kliu
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Mutant Allele(s):
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Zcwpw1em1Kliu/Zcwpw1em1Kliu
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Age:
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postnatal day 8
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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6H
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Expression in about 20% as many oocytes as in wild type ovaries.
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Analysis Tools