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Gene Expression Data
Assay Details
Assay
Reference: J:278517 Li M, et al., The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. Sci Adv. 2019;5(8):eaax1101
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6727338
Gene symbol: Mlh1
Gene name: mutL homolog 1
Antibody: anti-MLH-1 (550838)
Detection system: Alexa Fluor 488
Results
Specimen 3U: postnatal day 35 (more )
Note: Pachytene stage. Double labeled: green - Mlh1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 3U Foci of expression in spermatocytes indicating sites of crossovers.
Specimen 3V: postnatal day 35; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Pachytene-like. Double labeled: green - Mlh1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Absent 3V No foci of expression were found.
Specimen 5W: embryonic day 17.5 (more )
Note: Pachytene stage. Double labeled: green - Mlh1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS25: oocyte Present Regionally restricted 5W
Specimen 5X: embryonic day 17.5; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Pachytene stage. Double labeled: green - Mlh1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS25: oocyte Present Regionally restricted 5X Number of foci of expression similar to that of +/+ oocytes at pachytene stage; no significant differences were observed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory