Sycp1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:278517 Li M, et al., The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. Sci Adv. 2019;5(8):eaax1101
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6727333
Gene symbol:	Sycp1
Gene name:	synaptonemal complex protein 1

Antibody:	anti-Sycp1 (A12139)
Detection system:	Alexa Fluor 488

Results

3I ©

3I' ©

Specimen 3I/3I': postnatal day 25 (more

)
Note: Zygotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	3I , 3I'	Expression as dimers localized in central element (CE).

3J ©

3J' ©

Specimen 3J/3J': postnatal day 25; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Zygotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	3J' , 3J	Expression in central element on some chromosomes - ones that are fully synapsed.

3K ©

3K' ©

Specimen 3K/3K': postnatal day 25 (more

)
Note: Pachytene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	3K' , 3K	Expression as dimers localized in central element (CE).

3L ©

3L' ©

Specimen 3L/3L': postnatal day 25; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Leptotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	3L , 3L'	No apparent difference in expression pattern in synapsed chromosomes. No expression in unsynapsed chromosomes.

5I ©

5I' ©

Specimen 5I/5I': embryonic day 17.5 (more

)
Note: Zygotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present	Regionally restricted	5I' , 5I

5J ©

5J' ©

Specimen 5J/5J': embryonic day 17.5; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Zygotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present	Regionally restricted	5J , 5J'	Expression localized in central element (CE) of pair chromosomes at zygotene stage.

5K ©

5K' ©

Specimen 5K/5K': embryonic day 17.5 (more

)
Note: Pachytene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present	Regionally restricted	5K' , 5K

5L ©

5L' ©

Specimen 5L/5L': embryonic day 17.5; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Pachytene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present	Regionally restricted	5L , 5L'	Expression localized in central element (CE) of pair chromosomes at pachtene stage.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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