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Gene Expression Data
Assay Details
Assay
Reference: J:297659 Ge Y, et al., Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins. Dev Dyn. 2020 Aug 2;
Assay type: Recombinase reporter
MGI Accession ID: MGI:6727042
Gene symbol: Atoh7
Gene name: atonal bHLH transcription factor 7
Reporter: tdTomato
Direct Detection
Assay notes: The pregnant mice were intraperitoneally injected of tamoxifen with different doses (0, 4, 8, 80 mg/kg) at E12.5 and assayed at either E14.5, P0 or P16.
Results
Specimen Fig6D E14.5 0mg/kg: embryonic day 14.5; Atoh7tm3(cre/ERT2)Xmu/Atoh7+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. no tamoxifen
Structure Level Pattern Image Note
TS22: retina Trace 6D only very scarce tdTomatopositive cells could be seen, indicating that there was only a very low level of leaky activity of CreERT2
Specimen Fig6E E14.5 4mg/kg: embryonic day 14.5; Atoh7tm3(cre/ERT2)Xmu/Atoh7+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 4mg/kg tamoxifen
Structure Level Pattern Image Note
TS22: retina Present 6E
Specimen Fig6F E14.5 8mg/kg: embryonic day 14.5; Atoh7tm3(cre/ERT2)Xmu/Atoh7+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 8mg/kg tamoxifen
Structure Level Pattern Image Note
TS22: retina Present 6F
Specimen Fig6G E14.5 80mg/kg: embryonic day 14.5; Atoh7tm3(cre/ERT2)Xmu/Atoh7+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 80mg/kg tamoxifen
Structure Level Pattern Image Note
TS22: retina Strong Widespread 6G most cells in the ganglion cell layer (GCL) are positive
Specimen Fig6J P0 0mg/kg: postnatal day 0; Atoh7tm3(cre/ERT2)Xmu/Atoh7+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. no tamoxifen
Structure Level Pattern Image Note
TS27: retina Trace 6J only very scarce tdTomatopositive cells could be seen, indicating that there was only a very low level of leaky activity of CreERT2
Specimen Fig6K P0 4mg/kg: postnatal day 0; Atoh7tm3(cre/ERT2)Xmu/Atoh7+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 4mg/kg tamoxifen
Structure Level Pattern Image Note
TS27: retina Present 6K
Specimen Fig6L P0 8mg/kg: postnatal day 0; Atoh7tm3(cre/ERT2)Xmu/Atoh7+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 8mg/kg tamoxifen
Structure Level Pattern Image Note
TS27: retina Present 6L
Specimen Fig6M P0 80mg/kg: postnatal day 0; Atoh7tm3(cre/ERT2)Xmu/Atoh7+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 80mg/kg tamoxifen
Structure Level Pattern Image Note
TS27: retina Strong Widespread 6M
Specimen Fig6N P16 80mg/kg: postnatal day 16; Atoh7tm3(cre/ERT2)Xmu/Atoh7+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 80mg/kg tamoxifen
Structure Level Pattern Image Note
TS28: retina Strong Widespread 6N
Specimen Fig6O P16 80mg/kg: postnatal day 16; Atoh7tm3(cre/ERT2)Xmu/Atoh7+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. 80mg/kg tamoxifen
Structure Level Pattern Image Note
TS28: retina Present 6O in the other layers included photoreceptors, cones, horizontal cells, amacrine cells, and RGCs
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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