Ngfr Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:290227 Urness LD, et al., Slc26a9(P2ACre) : a new CRE driver to regulate gene expression in the otic placode lineage and other FGFR2b-dependent epithelia. Development. 2020 Jul 8;147(13):dev191015
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6725839
Gene symbol:	Ngfr
Gene name:	nerve growth factor receptor (TNFR superfamily, member 16)

Antibody:	anti-p75^NTR (#07-476)
Detection system:	Secondary antibody coupled to DyLight 594

Results

7B left base ©

Specimen 7B Fgf8^-/c base: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon (more

)
Note: Also labeled with phalloidin (green).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	7B left base	Expression is present in the inner pillar cells.

7B center base ©

Specimen 7B Fgf8^-/c;Slc26a9^P2ACre/+ base: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Slc26a9^em1(cre)Sms/Slc26a9⁺ (more

)
Note: Conditional mutant. Also labeled with phalloidin (green).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	7B center base	Expression is present in the inner pillar cells; there are gaps in staining at the epithelial surface, indicative of incomplete inner pillar cell differentiation.

7B right base ©

Specimen 7B Fgf8^-/c;Pax2-cre/0 base: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant. Also labeled with phalloidin (green).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	7B right base	Expression is present in the inner pillar cells; there are gaps in staining at the epithelial surface, indicative of incomplete inner pillar cell differentiation.

7B left mid ©

Specimen 7B Fgf8^-/c mid: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon (more

)
Note: Also labeled with phalloidin (green).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	7B left mid	Expression is present in the inner pillar cells.

7B center mid ©

Specimen 7B Fgf8^-/c;Slc26a9^P2ACre/+ mid: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Slc26a9^em1(cre)Sms/Slc26a9⁺ (more

)
Note: Conditional mutant. Also labeled with phalloidin (green).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	7B center mid	Expression is present in the inner pillar cells; there are gaps in staining at the epithelial surface, indicative of incomplete inner pillar cell differentiation.

7B right mid ©

Specimen 7B Fgf8^-/c;Pax2-cre/0 mid: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant. Also labeled with phalloidin (green).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	7B right mid	Expression is present in the inner pillar cells; there are gaps in staining at the epithelial surface, indicative of incomplete inner pillar cell differentiation.

7B left apex ©

Specimen 7B Fgf8^-/c apex: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon (more

)
Note: Also labeled with phalloidin (green).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	7B left apex	Expression is present in the inner pillar cells.

7B center apex ©

Specimen 7B Fgf8^-/c;Slc26a9^P2ACre/+ apex: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Slc26a9^em1(cre)Sms/Slc26a9⁺ (more

)
Note: Conditional mutant. Also labeled with phalloidin (green).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	7B center apex	Expression is present in the inner pillar cells; there are gaps in staining at the epithelial surface, indicative of incomplete inner pillar cell differentiation.

7B right apex ©

Specimen 7B Fgf8^-/c;Pax2-cre/0 apex: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant. Also labeled with phalloidin (green).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	7B right apex	Expression is present in the inner pillar cells; there are gaps in staining at the epithelial surface, indicative of incomplete inner pillar cell differentiation.

Specimen not shown Fgf8^c/+: embryonic day 18.5; Fgf8^tm2Moon/Fgf8⁺ (more

)

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted		Expression is seen in inner pillar cells; there is continuous basal-to-apical expression.

Specimen not shown Fgf8^c/+;Slc26a9^P2ACre/+: embryonic day 18.5; Fgf8^tm2Moon/Fgf8⁺, Slc26a9^em1(cre)Sms/Slc26a9⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted		Expression is seen in inner pillar cells; there is continuous basal-to-apical expression.

S1_left base ©

Specimen S1 Fgf8^-/c base: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon (more

)
Note: Double labeled: red - Ngfr; green - Myo7a (assay MGI:6725840); also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	S1_left base	Expression is present in the inner pillar cell; this cell extends beyond the level of the hair cells.

S1_center base ©

Specimen S1 Fgf8^-/c;Slc26a9^P2ACre/+ base: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Slc26a9^em1(cre)Sms/Slc26a9⁺ (more

)
Note: Conditional mutant. Double labeled: red - Ngfr; green - Myo7a (assay MGI:6725840); also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	S1_center base	Expression is present in the inner pillar cell; this cell extends beyond the level of the hair cells.

S1_right base ©

Specimen S1 Fgf8^-/c;Pax2-cre/0 base: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant. Double labeled: red - Ngfr; green - Myo7a (assay MGI:6725840); also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	S1_right base	Expression is present in the inner pillar cell; this cell extends beyond the level of the hair cells.

S1_left mid ©

Specimen S1 Fgf8^-/c mid: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon (more

)
Note: Double labeled: red - Ngfr; green - Myo7a (assay MGI:6725840); also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	S1_left mid	Expression is present in the inner pillar cell; this cell extends beyond the level of the hair cells.

Specimen S1 Fgf8^-/c;Slc26a9^P2ACre/+ mid: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Slc26a9^em1(cre)Sms/Slc26a9⁺ (more

)
Note: Conditional mutant. Double labeled: red - Ngfr; green - Myo7a (assay MGI:6725840); also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	S1_center mid	Expression is present in the inner pillar cell; in the mutant in this area this cell does not extends beyond the level of the hair cells, narrowing the distance between the inner hair cell and the first outer hair cell.

S1_right mid ©

Specimen S1 Fgf8^-/c;Pax2-cre/0 mid: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant. Double labeled: red - Ngfr; green - Myo7a (assay MGI:6725840); also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	S1_right mid	Expression is present in the inner pillar cell; in the mutant in this area this cell does not extends beyond the level of the hair cells, narrowing the distance between the inner hair cell and the first outer hair cell.

S1_left apex ©

Specimen S1 Fgf8^-/c apex: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon (more

)
Note: Double labeled: red - Ngfr; green - Myo7a (assay MGI:6725840); also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	S1_left apex	Expression is present in the inner pillar cell; this cell extends beyond the level of the hair cells.

Specimen S1 Fgf8^-/c;Slc26a9^P2ACre/+ apex: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Slc26a9^em1(cre)Sms/Slc26a9⁺ (more

)
Note: Conditional mutant. Double labeled: red - Ngfr; green - Myo7a (assay MGI:6725840); also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	S1_center apex	Expression is present in the inner pillar cell; in the mutant in this area this cell does not extends beyond the level of the hair cells, narrowing the distance between the inner hair cell and the first outer hair cell.

Specimen S1 Fgf8^-/c;Pax2-cre/0 apex: embryonic day 18.5; Fgf8^tm1Mrc/Fgf8^tm2Moon, Tg(Pax2-cre)1Akg/0 (more

)
Note: Conditional mutant. Double labeled: red - Ngfr; green - Myo7a (assay MGI:6725840); also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	S1_right apex	Expression is present in the inner pillar cell; in the mutant in this area this cell does not extends beyond the level of the hair cells, narrowing the distance between the inner hair cell and the first outer hair cell.

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