Ddx4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:241010 Mu W, et al., EZH1 in germ cells safeguards the function of PRC2 during spermatogenesis. Dev Biol. 2017 Apr 15;424(2):198-207
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6721113
Gene symbol:	Ddx4
Gene name:	DEAD box helicase 4

Antibody:	anti-MVH
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

2D ©

Specimen 2D: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: germ cell of testis	Present		2D

2F ©

Specimen 2F: postnatal day 7 (more

)
Note: Double labeled: red - Ddx4; green - Pcna (assay MGI:6721114).

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present		2F

4A Ctrl-1 ©

Specimen 4A Ctrl1: postnatal day 13; Ezh1^tm1Jnw/Ezh1⁺, Ezh2^tm1Tara/Ezh2⁺, Tg(Ddx4-cre)1Dcas/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A Ctrl-1
TS28: spermatogonium	Present		4A Ctrl-1

4A Ctrl-2 ©

Specimen 4A Ctrl2: postnatal day 13; Ezh1^tm1Jnw/Ezh1⁺, Ezh2^tm1Tara/Ezh2⁺, Tg(Ddx4-cre)1Dcas/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A Ctrl-2
TS28: spermatogonium	Present		4A Ctrl-2

4A DKO-1 ©

Specimen 4A DKO1: postnatal day 13; Ezh1^tm1Jnw/Ezh1^tm1Jnw, Ezh2^tm1Tara/Ezh2^tm1Tara, Tg(Ddx4-cre)1Dcas/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present		4A DKO-1
TS28: spermatocyte	Present		4A DKO-1	Meiotic arrest at pachytene stage.

4A DKO-2 ©

Specimen 4A DKO2: postnatal day 13; Ezh1^tm1Jnw/Ezh1^tm1Jnw, Ezh2^tm1Tara/Ezh2^tm1Tara, Tg(Ddx4-cre)1Dcas/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		4A DKO-2	Meiotic arrest at pachytene stage.
TS28: spermatogonium	Present		4A DKO-2

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24