Probe:	Met cDNA12
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: brainstem	Present	Regionally restricted	1a , S1d	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), nucleus of the hypoglossal nerve (nXII), as well as the nucleus of the facial nerve (nVII).
TS21: brainstem nucleus	Present	Regionally restricted	1a , S1d	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), nucleus of the hypoglossal nerve (nXII), as well as the nucleus of the facial nerve (nVII).
TS21: facial nucleus	Present		S1d , 1a

Structure	Level	Pattern	Image	Note
TS21: brainstem	Present	Regionally restricted	S1e , 1b	Expression is in subsets of cranial motor nuclei: in and lateral to the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), nucleus of the hypoglossal nerve (nXII), as well as the nucleus of the facial nerve (nVII).
TS21: brainstem nucleus	Present	Regionally restricted	S1e , 1b	Expression is in subsets of cranial motor nuclei: in and lateral to the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), nucleus of the hypoglossal nerve (nXII), as well as the nucleus of the facial nerve (nVII).
TS21: facial nucleus	Present		S1e , 1b

Structure	Level	Pattern	Image	Note
TS21: brainstem	Present	Regionally restricted	S1f , 1c	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), nucleus of the hypoglossal nerve (nXII), as well as the nucleus of the facial nerve (nVII).
TS21: brainstem nucleus	Present	Regionally restricted	S1f , 1c	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), nucleus of the hypoglossal nerve (nXII), as well as the nucleus of the facial nerve (nVII).
TS21: midbrain roof plate	Present	Regionally restricted	1c	Expression is in the superior colliculus.
TS21: facial nucleus	Present		1c

Structure	Level	Pattern	Image	Note
TS23: brainstem	Present	Regionally restricted	1d	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS23: brainstem nucleus	Present	Regionally restricted	1d	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS23: facial nucleus	Weak		1d

Structure	Level	Pattern	Image	Note
TS23: brainstem	Present	Regionally restricted	1e	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS23: brainstem nucleus	Present	Regionally restricted	1e	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS23: facial nucleus	Weak		1e

Structure	Level	Pattern	Image	Note
TS23: brainstem	Present	Regionally restricted	1f	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS23: brainstem nucleus	Present	Regionally restricted	1f	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS23: facial nucleus	Weak		1f

Structure	Level	Pattern	Image	Note
TS25: brainstem	Present	Regionally restricted	1g	Expression is in subsets of cranial motor nuclei: in and lateral to the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS25: brainstem nucleus	Present	Regionally restricted	1g	Expression is in subsets of cranial motor nuclei: in and lateral to the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS25: facial nucleus	Weak		1g

Structure	Level	Pattern	Image	Note
TS25: brainstem	Present	Regionally restricted	1h	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS25: brainstem nucleus	Present	Regionally restricted	1h	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS25: facial nucleus	Weak		1h

Structure	Level	Pattern	Image	Note
TS25: brainstem	Present	Regionally restricted	1i	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS25: brainstem nucleus	Present	Regionally restricted	1i	Expression is in subsets of cranial motor nuclei: the dorsal motor nucleus of the tenth nerve, nucleus ambiguous (the motor nucleus of the spinal accessory, XI, glossopharyngeal, IX, and vagus nerves, X), and nucleus of the hypoglossal nerve (nXII).
TS25: facial nucleus	Weak		1i

Structure	Level	Pattern	Image	Note
TS23: forebrain	Present		6g

Structure	Level	Pattern	Image	Note
TS25: forebrain	Present		6h

Structure	Level	Pattern	Image	Note
TS26: forebrain	Present		6i

Structure	Level	Pattern	Image	Note
TS21: brainstem nucleus	Present	Regionally restricted	S1g	Expression was either very weak or absent in neurons residing in the oculomotor (nIII), trochlear (nIV) and trigeminal nuclei (nV).

Structure	Level	Pattern	Image	Note
TS21: brainstem nucleus	Present	Regionally restricted		Expression is in subsets of cranial motor nuclei: facial nerve (nVII), nucleus ambiguous, the dorsal motor nucleus of the tenth nerve, nucleus of the hypoglossal nerve (nXII), and the nucleus of the solitary tract.

Structure	Level	Pattern	Image	Note
TS22: brainstem nucleus	Present	Regionally restricted		Expression is in subsets of cranial motor nuclei: the nucleus ambiguous, the dorsal motor nucleus of the tenth nerve, nucleus of the hypoglossal nerve (nXII), and the nucleus of the solitary tract, and in Barrington's nuclei, and is weak in the facial nerve (nVII).
TS22: midbrain tegmentum	Present	Regionally restricted		Expression is in the dorsal raphe nucleus.

Structure	Level	Pattern	Image	Note
TS25: brainstem nucleus	Present	Regionally restricted		Expression is in subsets of cranial motor nuclei: the nucleus ambiguous, the dorsal motor nucleus of the tenth nerve, nucleus of the hypoglossal nerve (nXII), and the nucleus of the solitary tract, and in Barrington's nuclei, and is weak in the facial nerve (nVII).
TS25: midbrain tegmentum	Present	Regionally restricted		Expression is in the dorsal raphe nucleus.

Structure	Level	Pattern	Image	Note
TS26: brainstem nucleus	Weak	Regionally restricted		Expression is in subsets of cranial motor nuclei: the nucleus ambiguous, the dorsal motor nucleus of the tenth nerve, nucleus of the hypoglossal nerve (nXII), and the nucleus of the solitary tract, and in Barrington's nuclei, and is absent in the facial nerve (nVII).
TS26: midbrain tegmentum	Weak	Regionally restricted		Expression is in the dorsal raphe nucleus.
TS26: hypoglossal nucleus	Weak

Structure	Level	Pattern	Image	Note
TS27: dorsal raphe nucleus	Present
TS27: brainstem nucleus	Weak	Regionally restricted		Expression is weak in Barrington's nuclei.
TS27: dorsal motor nucleus of vagus nerve	Weak	Regionally restricted
TS27: hypoglossal nucleus	Weak	Regionally restricted
TS27: nucleus ambiguus	Weak	Regionally restricted
TS27: solitary tract nucleus	Weak	Regionally restricted
TS27: facial nucleus	Absent