About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:126326 Olive V, et al., PU.1 (Sfpi1), a pleiotropic regulator expressed from the first embryonic stages with a crucial function in germinal progenitors. Development. 2007 Nov;134(21):3815-25
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6716855
Gene symbol: Spi1
Gene name: Spi-1 proto-oncogene
Antibody: anti-PU.1 (T-21): sc-352
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 2A: postnatal month 9 (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 2A Expressed in a small number of spermatogonia in the most peripheral region of the tubule, whereas the bulk of differentiated spermatogonia were negative.
TS28: secondary spermatocyte Present 2A Expressed in meiotic spermatocytes.
Specimen 2C: postnatal week 3 (more )
Structure Level Pattern Image Note
TS28: secondary spermatocyte Strong Uniform 2C
Specimen 2D: postnatal week 3 (more )
Structure Level Pattern Image Note
TS28: secondary spermatocyte Strong Uniform 2D
Specimen 2E: postnatal week 3 (more )
Structure Level Pattern Image Note
TS28: secondary spermatocyte Strong Uniform 2E
Specimen 2G: postnatal week 3 (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present 2G Expressed in single cells in the peripheral compartment of the seminiferous tubule.
Specimen 2H: postnatal week 3 (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present 2H Expressed in paired cells in the peripheral compartment of the seminiferous tubule.
Specimen 2I: postnatal week 3 (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present 2I Expressed in aligned cells in the peripheral compartment of the seminiferous tubule.
Specimen 3A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: germ cell of testis Present 3A
Specimen 3B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: germ cell of testis Present 3B
Specimen 3C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: germ cell of testis Present 3C
Specimen 3D: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: germ cell of testis Present 3D
Specimen 3E: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present 3E
Specimen 3F: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present 3F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory