Gene Expression Data
Assay Details
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Assay
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Reference:
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J:64996
Burgess RW, et al., Agrin isoforms with distinct amino termini. Differential expression, localization, and function. J Cell Biol. 2000 Oct 2;151(1):41-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6714281
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Gene symbol:
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Nefh
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Gene name:
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neurofilament, heavy polypeptide
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Antibody:
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anti-NF200
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed. Anti-NF200 MGI:6714230 may have been used in this assay.
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Results
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Specimen
8A:
embryonic day 18.0
(more  )
Specimen
8A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: diaphragm |
Present |
Regionally restricted |
8A
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Expression in motor axons forming a central nerve trunk; individual axons branch and terminate on myotubes.
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Specimen
8B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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AgrnGt(Ex192)Byg/AgrnGt(Ex192)Byg
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: diaphragm |
Weak |
Regionally restricted |
8B
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Expression in intramuscular nerve trunks, but motor axons sprout abnormally and form few nerve terminals.
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Analysis Tools
