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Gene Expression Data
Assay Details
Assay
Reference: J:301247 Probst S, et al., Spatiotemporal sequence of mesoderm and endoderm lineage segregation during mouse gastrulation. Development. 2021 Jan 7;148(1):dev193789
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6712844
Gene symbol: Eomes
Gene name: eomesodermin
Reporter: GFP
Direct Detection
Results
Specimen 1A: embryonic day 6.25; Eomestm2.1Sjar/Eomes+ (more )
Note: Triple labeled: green - Eomes reporter (nuclear GFP); red - Eomes reporter (membrane-bound Tomato; assay MGI:6712845); blue - Dapi.
Structure Level Pattern Image Note
TS9: embryo ectoderm Present Regionally restricted 1A Expression in posterior half of proximal epiblast before formation of the primitive streak.
Specimen 1B: embryonic day 6.75; Eomestm2.1Sjar/Eomes+ (more )
Note: Triple labeled: green - Eomes reporter (nuclear GFP); red - Eomes reporter (membrane-bound Tomato; assay MGI:6712845); blue - Dapi.
Structure Level Pattern Image Note
TS10: embryo ectoderm Present Regionally restricted 1B Expression in posterior epiblast.
TS10: embryo mesoderm Present Ubiquitous 1B Expression in all cells in the mesoderm layer - contains both mesoderm and definitive endoderm precursors.
Specimen 1C: embryonic day 7.25; Eomestm2.1Sjar/Eomes+ (more )
Note: Triple labeled: green - Eomes reporter (nuclear GFP); red - Eomes reporter (membrane-bound Tomato; assay MGI:6712845); blue - Dapi.
Structure Level Pattern Image Note
TS11: embryo ectoderm Present Regionally restricted 1C Expression in posterior epiblast.
TS11: embryo mesoderm Present Ubiquitous 1C Expression in all cells in the mesoderm layer - contains both mesoderm and definitive endoderm precursors.
TS11: embryonic visceral endoderm Weak 1C Expression down-regulated in embryonic visceral endoderm.
Specimen 1D: embryonic day 7.5; Eomestm2.1Sjar/Eomes+ (more )
Note: Triple labeled: green - Eomes reporter (nuclear GFP); red - Eomes reporter (membrane-bound Tomato; assay MGI:6712845); blue - Dapi.
Structure Level Pattern Image Note
TS11: embryo ectoderm Present Regionally restricted 1D Expression in posterior epiblast persisted until E7.5.
TS11: embryo mesoderm Present Regionally restricted 1D Expression in mesoderm wings that have migrated to anterior.
TS11: definitive endoderm Present Regionally restricted 1D Expression in definitive endoderm cells integrated into outer endoderm layer.
Specimen 1E: embryonic day 6.75; Eomestm2.1Sjar/Eomes+ (more )
Note: Triple labeled: green - Eomes reporter (nuclear GFP); red - Eomes reporter (membrane-bound Tomato; assay MGI:6712845); blue - Dapi.
Structure Level Pattern Image Note
TS10: embryo mesoderm Present 1E Expression in nascent mesoderm layer.
Specimen 1F: embryonic day 7.5; Eomestm2.1Sjar/Eomes+ (more )
Note: Triple labeled: green - Eomes reporter (nuclear GFP); red - Eomes reporter (membrane-bound Tomato; assay MGI:6712845); blue - Dapi.
Structure Level Pattern Image Note
TS11: definitive endoderm Present Widespread 1F Expression in endoderm layer, mainly consisting of epiblast-derived definitive endoderm cells. Only a few non-expressing cells detected.
TS11: embryonic visceral endoderm Weak 1F Expression down-regulated in embryonic visceral endoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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