Gene Expression Data
Assay Details
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Assay
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Reference:
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J:304806
Wray S, et al., Evidence that cells expressing luteinizing hormone-releasing hormone mRNA in the mouse are derived from progenitor cells in the olfactory placode. Proc Natl Acad Sci U S A. 1989 Oct;86(20):8132-6
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6711499
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Probe:
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LHRH probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
E11.5 not shown:
embryonic day 11.5
(more  )
Specimen
E11.5 not shown:
(close  )
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Genetic Background:
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NIH Swiss
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: nasal pit epithelium |
Present |
Regionally restricted |
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Expression first detected on E11.5 in cells of olfactory pit. Expression in cells in medial wall of olfactory pit epithelium, adjacent to nasal cavity, from E11.5 to E14.5.
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| TS19: brain |
Absent |
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Specimen
1A:
embryonic day 12.5
(more )
Specimen
1A:
(close )
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Genetic Background:
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NIH Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: olfactory epithelium |
Strong |
Regionally restricted |
1A
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Expression in cells in medial wall of olfactory epithelium, adjacent to nasal cavity, from E11.5 to E14.5. Intensity of expression in olfactory epitheilum increased between E11,5 and E12.5.
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| TS20: nose |
Present |
Regionally restricted |
1A
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From E12.5-E14.5, expression in cells directly adjacent to olfactory pit.
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Specimen
1B:
embryonic day 12.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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NIH Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: nose |
Present |
Regionally restricted |
1B
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From E12.5, expression in cells extending dorsally away from nasal cavity/olfactory pit.
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Specimen
E12.5 not shown:
embryonic day 12.5
(more )
Specimen
E12.5 not shown:
(close )
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Genetic Background:
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NIH Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
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Expression first detected within rostral telencephalon on E12.5.
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Specimen
E13.5 not shown:
embryonic day 13.5
(more )
Specimen
E13.5 not shown:
(close )
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Genetic Background:
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NIH Swiss
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: preoptic area |
Present |
Regionally restricted |
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Expression first detected in rostral preoptic area on E13.5.
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Specimen
2B:
embryonic day 14.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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NIH Swiss
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: nasal septum |
Present |
Clusters |
2B
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Expression in cells extending dorsally away from nasal cavity/olfactory pit. Expression in located bilaterally in cells clustered on "tracks" extending throughout nasal septum.
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Specimen
3A:
embryonic day 14.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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NIH Swiss
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: nose |
Present |
Regionally restricted |
3A
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Expression in cells on tracks in nasal region converging at junction between base of telencephalic hemisphere and olfactory bulb.
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| TS22: forebrain |
Present |
Regionally restricted |
3A
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Expression in cells in forebrain directly opposite point of convergence and within more caudal structures.
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Specimen
E14.5 not shown:
embryonic day 14.5
(more )
Specimen
E14.5 not shown:
(close )
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Genetic Background:
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NIH Swiss
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: preoptic area |
Present |
Regionally restricted |
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Expression first detected within caudal preoptic area/rostral diencephalon on E14.5.
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| TS22: olfactory epithelium |
Trace |
Single cells |
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By E14.5, only an occasional expressing cell detected in olfactory epithelium.
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Specimen
E15.5 not shown:
embryonic day 15.5
(more )
Specimen
E15.5 not shown:
(close )
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Genetic Background:
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NIH Swiss
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: diencephalon |
Present |
Regionally restricted |
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Expression first detected in caudal diencephalon on E15.5.
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| TS23: olfactory epithelium |
Absent |
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By E15.5, no expression in olfactory epithelium.
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Analysis Tools