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Gene Expression Data
Assay Details
Assay
Reference: J:111698 Fish JL, et al., Aspm specifically maintains symmetric proliferative divisions of neuroepithelial cells. Proc Natl Acad Sci U S A. 2006 Jul 5;103(27):10438-43
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6704895
Gene symbol: Aspm
Gene name: abnormal spindle microtubule assembly
Antibody: Aspm antibody
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1A: embryonic day 12.5 (more )
Note: Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
Structure Level Pattern Image Note
TS20: telencephalon Present Regionally restricted 1A Aspm , 1A Merge Expression is in the dorsal telencephalon and protein is concentrated at the poles of the mitotic spindle in neuroepithelial cells.
Specimen 1B: embryonic day 14.5; Btg2tm1Wbh/Btg2+ (more )
Note: Double labeled: white - Aspm; green - Btg2 reporter (assay MGI:6704898).
Structure Level Pattern Image Note
TS22: telencephalon Present Regionally restricted 1B Aspm Expression is associated with the spindle poles and GFP-negative neuroepithelial cells had stronger staining at spindle poles than did GFP-positive neuroepithelial cells.
Specimen 6 prometa: embryonic day 12.5; Btg2tm1Wbh/Btg2+ (more )
Note: Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
Structure Level Pattern Image Note
TS20: telencephalon Present 6_prometa Aspm , 6_prometa merge Expression is associated with the spindle poles during all phases of mitosis, with a decrease in the intensity of immunostaining in telophase in neuroepithelial cells.
Specimen 6 meta: embryonic day 12.5; Btg2tm1Wbh/Btg2+ (more )
Note: Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
Structure Level Pattern Image Note
TS20: telencephalon Present 6_meta merge , 6_meta Aspm Expression is associated with the spindle poles during all phases of mitosis, with a decrease in the intensity of immunostaining in telophase in neuroepithelial cells.
Specimen 6 telo: embryonic day 12.5; Btg2tm1Wbh/Btg2+ (more )
Note: Double labeled: red - Aspm; green - Tubg1 (assay MGI:6704896) and Tubg2 (assay MGI:6704980).
Structure Level Pattern Image Note
TS20: telencephalon Present 6_telo Aspm , 6_telo merge Expression is associated with the spindle poles during all phases of mitosis, with a decrease in the intensity of immunostaining in telophase in neuroepithelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/22/2024
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