Gene Expression Data
Assay Details
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Assay
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Reference:
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J:302594
Hall ET, et al., Cytoneme delivery of sonic hedgehog from ligand-producing cells requires Myosin 10 and a dispatched-BOC/CDON co-receptor complex. Elife. 2021 Feb 11;10:e61432
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6696109
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Reporter:
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GFP
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Antibody:
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anti-GFP (Aves)
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
5A:
embryonic day 9.5; Shhtm6Amc/Shh +
(more  )
Note: cardiac-level neural tube
Specimen
5A:
(close  )
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Genetic Background:
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B6.Cg-Shhtm6Amc
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Mutant Allele(s):
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Shhtm6Amc/Shh+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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cardiac-level neural tube
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Specimen
5B:
embryonic day 9.5; Myo10m1J/Myo10 +, Shhtm6Amc/Shh +
(more )
Note: cardiac-level neural tube
Specimen
5B:
(close )
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Genetic Background:
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B6.Cg-Shhtm6Amc Myo10m1J/GrsrJ
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Mutant Allele(s):
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Myo10m1J/Myo10+
Shhtm6Amc/Shh+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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cardiac-level neural tube
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Specimen
5C:
(close )
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Genetic Background:
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B6.Cg-Shhtm6Amc Myo10m1J/GrsrJ
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Mutant Allele(s):
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Myo10m1J/Myo10m1J
Shhtm6Amc/Shh+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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cardiac-level neural tube
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Specimen
5H:
(close )
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Genetic Background:
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B6.Cg-Shhtm6Amc Myo10m1J/GrsrJ
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Mutant Allele(s):
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Myo10m1J/Myo10+
Shhtm6Amc/Shh+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
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5H
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Regression has been initiated.
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Specimen
5I:
(close )
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Genetic Background:
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B6.Cg-Shhtm6Amc Myo10m1J/GrsrJ
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Mutant Allele(s):
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Myo10m1J/Myo10m1J
Shhtm6Amc/Shh+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
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5I
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Regression is delayed in the mutants.
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Analysis Tools
