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Gene Expression Data
Assay Details
Assay
Reference: J:231231 Abby E, et al., Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts. Nat Commun. 2016;7:10324
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6695761
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4/MVH (ab27591)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Two antibodies were described MGI:5315099 and MGI:6690812, but the one used in this assay was not detailed; either could have been used.
Results
Specimen 3d +/+: embryonic day 18.5 (more )
Note: Double labeled: magenta - Ddx4; brown - Casp3 (assay MGI:6695760).
Structure Level Pattern Image Note
TS26: germ cell of ovary Present 3d +/+
Specimen 3d -/-: embryonic day 18.5; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Casp3 (assay MGI:6695760).
Structure Level Pattern Image Note
TS26: germ cell of ovary Present 3d -/-
Specimen 4a +/+ VASA/OCT4: embryonic day 14.5 (more )
Note: Double labeled: magenta - Ddx4; brown - Pou5f1 (assay MGI:6705056).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 4a Meioc+/+ Vasa/Oct4
Specimen 4a -/- VASA/OCT4: embryonic day 14.5; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Pou5f1 (assay MGI:6705056).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 4a Meioc-/- Vasa/Oct4
Specimen 4a +/+ VASA/STRA8: embryonic day 14.5 (more )
Note: Double labeled: magenta - Ddx4; brown - Stra8 (assay MGI:6705058).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 4a Meioc+/+ Vasa/Stra8
Specimen 4a -/- VASA/STRA8: embryonic day 14.5; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Stra8 (assay MGI:6705058).
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 4a Meioc-/- Vasa/Stra8
Specimen 4a +/+ VASA/BrdU: embryonic day 14.5 (more )
Note: Double labeled: magenta - Ddx4; brown - BrdU.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 4a Meioc+/+ Vasa/BrdU
Specimen 4a -/- VASA/BrdU: embryonic day 14.5; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - BrdU.
Structure Level Pattern Image Note
TS22: germ cell of ovary Present 4a Meioc-/- Vasa/BrdU
Specimen 4e +/+ VASA/g-H2AX: embryonic day 18.5 (more )
Note: Double labeled: magenta - Ddx4; brown - H2ax (assay MGI:6715676).
Structure Level Pattern Image Note
TS26: germ cell of ovary Present 4e Meioc+/+ Vasa/gamma-H2AX
Specimen 4e -/- VASA/g-H2AX: embryonic day 18.5; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - H2ax (assay MGI:6715676).
Structure Level Pattern Image Note
TS26: germ cell of ovary Present 4e Meioc-/- Vasa/gamma-H2AX
Specimen 4e +/+ VASA/STRA8: embryonic day 18.5 (more )
Note: Double labeled: magenta - Ddx4; brown - Stra8 (assay MGI:6705058).
Structure Level Pattern Image Note
TS26: germ cell of ovary Present 4e Meioc+/+ Vasa/Stra8
Specimen 4e -/- VASA/STRA8: embryonic day 18.5; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Stra8 (assay MGI:6705058).
Structure Level Pattern Image Note
TS26: germ cell of ovary Present 4e Meioc-/- Vasa/Stra8
Specimen 5a +/+ VASA/SYCP3 18.5-dpc: embryonic day 18.5 (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 5a 18.5dpc Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 18.5-dpc: embryonic day 18.5; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 5a 18.5dpc Meioc-/- Vasa/Sycp3
Specimen 5a +/+ VASA/SYCP3 6-dpp: postnatal day 6 (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 6dpp Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 6-dpp: postnatal day 6; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 6dpp Meioc-/- Vasa/Sycp3
Specimen 5a +/+ VASA/SYCP3 8-dpp: postnatal day 8 (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 8dpp Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 8-dpp: postnatal day 8; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 8dpp Meioc-/- Vasa/Sycp3
Specimen 5a +/+ VASA/SYCP3 10-dpp: postnatal day 10 (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 10dpp Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 10-dpp: postnatal day 10; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 10dpp Meioc-/- Vasa/Sycp3
Specimen 5a +/+ VASA/SYCP3 16-dpp: postnatal day 16 (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 16dpp Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 16-dpp: postnatal day 16; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 16dpp Meioc-/- Vasa/Sycp3
Specimen 5a +/+ VASA/SYCP3 20-dpp: postnatal day 20 (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 20dpp Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 20-dpp: postnatal day 20; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 20dpp Meioc-/- Vasa/Sycp3
Specimen 5a +/+ VASA/SYCP3 Adult: postnatal adult (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a Adult Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 Adult: postnatal adult; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: magenta - Ddx4; brown - Sycp3 (assay MGI:6715942).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a Adult Meioc-/- Vasa/Sycp3
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory