Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:217303
Koudelka J, et al., Genetic dissection of TrkB activated signalling pathways required for specific aspects of the taste system. Neural Dev. 2014;9:21
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6693303
|
|
Gene symbol:
|
Krt8
|
|
Gene name:
|
keratin 8
|
|
Antibody:
|
TROMA 1
|
|
Detection system:
|
Alexa Fluor 555
|
|
|
Results
|
Specimen
2D W/W:
(close  )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm2Kln/Ntrk2tm2Kln
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2D P/P:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm1Mini/Ntrk2tm1Mini
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: fungiform papillae |
Strong |
Regionally restricted |
2D P/P
|
Increase in innervated taste buds, exceeding number in controls at this age.
|
Specimen
2D D/D:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm2.1Mini/Ntrk2tm2.1Mini
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: fungiform papillae |
Weak |
Regionally restricted |
2D D/D
|
Amount of innervated taste buds comparable to that seen at E16.5, likely reflecting a loss of geniculate neurons.
|
Specimen
2F +/+:
postnatal day 0
(more  )
Specimen
2F +/+:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2F S/S:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm1Kln/Ntrk2tm1Kln
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: fungiform papillae |
Weak |
Regionally restricted |
2F S/S
|
Significantly fewer innervated tastebuds when compared with controls.
|
Specimen
3B/C:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm2Kln/Ntrk2tm2Kln
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3D/E:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm1Mini/Ntrk2tm1Mini
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: fungiform papillae |
Present |
Regionally restricted |
3E
,
3D
|
Taste bud morphology not significantly different compared with controls.
|
Specimen
3F/G:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm2.1Mini/Ntrk2tm2.1Mini
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: fungiform papillae |
Weak |
Regionally restricted |
3G
,
3F
|
Taste buds morphologically different and significantly smaller compared with control and P/P mutants.
|
Specimen
3I/J:
postnatal day 0
(more )
Specimen
3I/J:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3K/L:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm1Kln/Ntrk2tm1Kln
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: fungiform papillae |
Weak |
Regionally restricted |
3L
,
3K
|
Reduction in taste bud size similar to that of D/D mutants.
|
Specimen
4B/C:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm2Kln/Ntrk2tm2Kln
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4D/E:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm1Mini/Ntrk2tm1Mini
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: fungiform papillae |
Present |
Regionally restricted |
4E
,
4D
|
No significant morphological differences compared with W/W controls.
|
Specimen
4G/H:
postnatal adult
(more )
Specimen
4G/H:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4I/J:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6
|
|
Mutant Allele(s):
|
Ntrk2tm1Kln/Ntrk2tm1Kln
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: fungiform papillae |
Present |
Regionally restricted |
4J
,
4I
|
No significant morphological differences compared with controls.
|
|
Analysis Tools