Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:92833
Hertzano R, et al., Transcription profiling of inner ears from Pou4f3(ddl/ddl) identifies Gfi1 as a target of the Pou4f3 deafness gene. Hum Mol Genet. 2004 Sep 15;13(18):2143-53
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6514450
|
|
Gene symbol:
|
Myo6
|
|
Gene name:
|
myosin VI
|
|
Antibody:
|
anti-myosin VI
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
|
Results
|
Specimen
3C:
embryonic day 18.5
(more  )
Note: Apex of cochlea
Specimen
3C:
(close  )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Apex of cochlea
|
Specimen
3D:
embryonic day 18.5
(more )
Note: Base of cochlea
Specimen
3D:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Base of cochlea
|
Specimen
3G:
(close )
|
Genetic Background:
|
C57BL/6J-Pou4f3ddl/J
|
|
Mutant Allele(s):
|
Pou4f3ddl/Pou4f3ddl
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Apex of cochlea
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
Present |
|
3G
|
Individual cells not readily distinguished.
|
Specimen
3H:
(close )
|
Genetic Background:
|
C57BL/6J-Pou4f3ddl/J
|
|
Mutant Allele(s):
|
Pou4f3ddl/Pou4f3ddl
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Base of cochlea
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
Present |
Regionally restricted |
3H
|
At base of cochlea, no inner hair cells were detected.
|
Specimen
3K:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
|
Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3tm1Xia
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Apex of cochlea
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
Present |
|
3K
|
Individual cells not readily distinguished.
|
Specimen
3L:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
|
Mutant Allele(s):
|
Pou4f3tm1Xia/Pou4f3tm1Xia
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Base of cochlea
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
Present |
Regionally restricted |
3L
|
At base of cochlea, no inner hair cells were detected.
|
Specimen
3O:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Gfi1tm1Tmo/Gfi1tm1Tmo
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Apex of cochlea
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
Present |
|
3O
|
Individual cells not readily distinguished.
|
Specimen
3P:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6
|
|
Mutant Allele(s):
|
Gfi1tm1Tmo/Gfi1tm1Tmo
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Base of cochlea
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea |
Present |
Regionally restricted |
3P
|
Inner hair cells present along entire length of cochlea.
|
|
Analysis Tools
