Map2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:296029 Zhou Z, et al., CAMSAP1 breaks the homeostatic microtubule network to instruct neuronal polarity. Proc Natl Acad Sci U S A. 2020 Sep 8;117(36):22193-22203
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6512958
Gene symbol:	Map2
Gene name:	microtubule-associated protein 2

Antibody:	anti-MAP2 (2a+2b; clone AP-20)
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. MAP2 antibody (H-300, SC-20172) MGI:6512918 may have been used in this assay.

Results

1D Map2 CAMSAP1 ©

Specimen 1D Map2 (left): postnatal day 0 (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	1D Map2 CAMSAP1	Expression in dendrites.

1D Map2 CAMSAP2 ©

Specimen 1D Map2 (middle): postnatal day 0 (more

)
Note: Triple labeled: red - Map2; green - Camsap2 (assay MGI:6512956); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	1D Map2 CAMSAP2	Expression in dendrites.

1D Map2 CAMSAP3 ©

Specimen 1D Map2 (right): postnatal day 0 (more

)
Note: Triple labeled: red - Map2; green - Camsap3 (assay MGI:6512957); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	1D Map2 CAMSAP3	Expression in dendrites.

3C (+/+) merge ©

Specimen 3C (+/+): embryonic day 18.5 (more

)
Note: Triple labeled: green - Map2; red - BrdU; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		3C (+/+) merge

3C (-/-) Merge ©

Specimen 3C (-/-): embryonic day 18.5; Camsap1^tm1Wem/Camsap1^tm1Wem (more

)
Note: Triple labeled: green - Map2; red - BrdU; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		3C (-/-) Merge

S1F (merge) ©

Specimen S1F: embryonic day 15.5 (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		S1F (merge)

S1G (merge) ©

Specimen S1G: embryonic day 17.5 (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present		S1G (merge)

S1H (merge) ©

Specimen S1H: postnatal day 0 (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		S1H (merge)

S4E (+/+) (merge) ©

S4E (+/+) Map2 ©

Specimen S4E (+/+): embryonic day 18.0 (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		S4E (+/+) Map2 , S4E (+/+) (merge)

Specimen S4E (-/-): embryonic day 18.0; Camsap1^tm1Wem/Camsap1^tm1Wem (more

)
Note: Triple labeled: red - Map2; green - Camsap1 (assay MGI:6512955); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		S4E (-/-) (merge) , S4E (-/-) Map2

Specimen S7A (+/+): postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		S7A (+/+) Map2 , S7A (+/+) Merged

Specimen S7A (-/-): postnatal day 0; Camsap1^tm1Wem/Camsap1^tm1Wem (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	S7A (-/-) Merged , S7A (-/-) Map2	Expression in cortical lamina fluctuated less than in (+/+) mice

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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