Gene Expression Data
Assay Details
Assay
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Reference:
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J:178989
Yvernogeau L, et al., Limb bud colonization by somite-derived angioblasts is a crucial step for myoblast emigration. Development. 2012 Jan;139(2):277-87
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6511026
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Gene symbol:
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Cd34
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Gene name:
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CD34 antigen
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Antibody:
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anti-Cd34
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Detection system:
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rhodamine
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Results
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Specimen
1C:
embryonic day 9.0; Kdrtm1Jrt/Kdr +, Pax3tm4Buck/Pax3 +
(more )
Note: Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
Specimen
1C:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+ Pax3tm4Buck/Pax3+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: forelimb bud |
Present |
Regionally restricted |
1C
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Expression in endothelial cells in presumptive forelimb region
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Specimen
1D:
embryonic day 9.0; Kdrtm1Jrt/Kdr +, Pax3tm4Buck/Pax3 +
(more )
Note: Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
Specimen
1D:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+ Pax3tm4Buck/Pax3+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: forelimb bud |
Present |
Regionally restricted |
1D
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Expression in endothelial cells in presumptive forelimb region
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Specimen
1G:
embryonic day 9.5; Kdrtm1Jrt/Kdr +, Pax3tm4Buck/Pax3 +
(more )
Note: Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
Specimen
1G:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Kdrtm1Jrt/Kdr+ Pax3tm4Buck/Pax3+
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Age:
|
embryonic day 9.5
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Sex:
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Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: forelimb bud |
Present |
Regionally restricted |
1G
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Expression in endothelial cells in presumptive forelimb region
|
Specimen
1H:
embryonic day 9.5; Kdrtm1Jrt/Kdr +, Pax3tm4Buck/Pax3 +
(more )
Note: Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
Specimen
1H:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Kdrtm1Jrt/Kdr+ Pax3tm4Buck/Pax3+
|
Age:
|
embryonic day 9.5
|
Sex:
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Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: forelimb bud |
Present |
Regionally restricted |
1H
|
Expression in endothelial cells in presumptive forelimb region
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Specimen
1K:
embryonic day 10.5; Kdrtm1Jrt/Kdr +, Pax3tm4Buck/Pax3 +
(more )
Note: Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
Specimen
1K:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Kdrtm1Jrt/Kdr+ Pax3tm4Buck/Pax3+
|
Age:
|
embryonic day 10.5
|
Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud |
Present |
Regionally restricted |
1K
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Expression in endothelial cells in presumptive forelimb region
|
Specimen
1L:
embryonic day 10.5; Kdrtm1Jrt/Kdr +, Pax3tm4Buck/Pax3 +
(more )
Note: Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
Specimen
1L:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Kdrtm1Jrt/Kdr+ Pax3tm4Buck/Pax3+
|
Age:
|
embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud |
Present |
Regionally restricted |
1L
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Expression in endothelial cells in presumptive forelimb region
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Specimen
1O:
embryonic day 11.5; Kdrtm1Jrt/Kdr +, Pax3tm4Buck/Pax3 +
(more )
Note: Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
Specimen
1O:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Kdrtm1Jrt/Kdr+ Pax3tm4Buck/Pax3+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
1O
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Expression in endothelial cells in presumptive forelimb region
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Specimen
1P:
embryonic day 11.5; Kdrtm1Jrt/Kdr +, Pax3tm4Buck/Pax3 +
(more )
Note: Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
Specimen
1P:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Kdrtm1Jrt/Kdr+ Pax3tm4Buck/Pax3+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud |
Present |
Regionally restricted |
1P
|
Expression in endothelial cells in presumptive forelimb region
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Specimen
4A:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax3tm4Buck/Pax3tm4Buck
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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Double labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: dorsal aorta |
Present |
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4A
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Expression in endothelial cells displaying a normal pattern in trunk.
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Specimen
4C:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax3tm4Buck/Pax3tm4Buck
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Age:
|
embryonic day 13.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Triple labeled: red - Cd34; green - Pax3 reporter (assay MGI:6511025); blue - alpha-SMA (Acta2 assay MGI:6511031).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forelimb |
Present |
Regionally restricted |
4C
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Expression in endothelial cells displaying a normal pattern in limb.
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