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Gene Expression Data
Assay Details
Assay
Reference: J:178989 Yvernogeau L, et al., Limb bud colonization by somite-derived angioblasts is a crucial step for myoblast emigration. Development. 2012 Jan;139(2):277-87
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6511025
Gene symbol: Pax3
Gene name: paired box 3
Reporter: GFP
Direct Detection
Results
Specimen 1B: embryonic day 9.0; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: 15 somites
Structure Level Pattern Image Note
TS14: trunk somite Present Regionally restricted 1B Expression in myogenic progenitor cells. Expression restricted to somites.
TS14: forelimb bud Absent 1B
Specimen 1C: embryonic day 9.0; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: Triple labeled: green - Pax3 reporter; red - Cd34 (assay MGI:6511026); blue - DAPI.
Structure Level Pattern Image Note
TS14: trunk somite Present 1C
Specimen 1D: embryonic day 9.0; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: Triple labeled: green - Pax3 reporter; red - Cd34 (assay MGI:6511026); blue - DAPI.
Structure Level Pattern Image Note
TS14: trunk somite Present 1D
Specimen 1F: embryonic day 9.5; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: 21 somites
Structure Level Pattern Image Note
TS15: trunk somite Present 1F
TS15: forelimb bud Absent 1F No expression in cells leaving the somite.
Specimen 1G: embryonic day 9.5; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: Triple labeled: green - Pax3 reporter; red - Cd34 (assay MGI:6511026); blue - DAPI.
Structure Level Pattern Image Note
TS15: trunk somite Present Regionally restricted 1G Expression in myogenic precursor cells delaminating from ventrolateral lip of dermomyotome.
Specimen 1H: embryonic day 9.5; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: Triple labeled: green - Pax3 reporter; red - Cd34 (assay MGI:6511026); blue - DAPI.
Structure Level Pattern Image Note
TS15: trunk somite Present Regionally restricted 1H Expression in myogenic precursor cells delaminating from ventrolateral lip of dermomyotome.
Specimen 1J: embryonic day 10.5; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: 33 somites
Structure Level Pattern Image Note
TS16: trunk somite Present 1J
TS16: forelimb bud Present Regionally restricted 1J Expression in myogenic cells in a dispersed pattern restricted to proximal zone.
Specimen 1K: embryonic day 10.5; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: Triple labeled: green - Pax3 reporter; red - Cd34 (assay MGI:6511026); blue - DAPI.
Structure Level Pattern Image Note
TS16: trunk somite Present 1K
TS16: forelimb bud Present Regionally restricted 1K Expression in myogenic cells in a dispersed pattern restricted to proximal zone.
Specimen 1L: embryonic day 10.5; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: Triple labeled: green - Pax3 reporter; red - Cd34 (assay MGI:6511026); blue - DAPI.
Structure Level Pattern Image Note
TS16: trunk somite Present 1L
TS16: forelimb bud Present Regionally restricted 1L Expression in myogenic cells in a dispersed pattern restricted to proximal zone.
Specimen 1N: embryonic day 11.5; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: 45 somites
Structure Level Pattern Image Note
TS19: trunk somite Present 1N
TS19: forelimb bud Present Regionally restricted 1N Expression in cells organized into dorsal and ventral muscular masses.
Specimen 1O: embryonic day 11.5; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: Triple labeled: green - Pax3 reporter; red - Cd34 (assay MGI:6511026); blue - DAPI.
Structure Level Pattern Image Note
TS19: forelimb bud Present Regionally restricted 1O Expression in cells organized into dorsal and ventral muscular masses.
Specimen 1P: embryonic day 11.5; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3+ (more )
Note: Triple labeled: green - Pax3 reporter; red - Cd34 (assay MGI:6511026); blue - DAPI.
Structure Level Pattern Image Note
TS19: forelimb bud Present Regionally restricted 1P Expression in cells organized into dorsal and ventral muscular masses.
Specimen 4A: embryonic day 11.5; Pax3tm4Buck/Pax3tm4Buck (more )
Note: Double labeled: green - Pax3 reporter; red - Cd34 (assay MGI:6511026).
Structure Level Pattern Image Note
TS19: neural tube Present Regionally restricted 4A Expression in dorsal neural tube.
TS19: dermomyotome Present 4A Expression in dermomyotome, which is drastically reduced.
TS19: forelimb bud Absent 4A Authors report total absence of myogenic cells in the forelimb.
Specimen 4B: embryonic day 11.5; Pax3tm4Buck/Pax3tm4Buck (more )
Note: Double labeled: green - Pax3 reporter; red - alpha-SMA (Acta2 assay MGI:6511029).
Structure Level Pattern Image Note
TS19: dorsal aorta Absent 4B
Specimen 4C: embryonic day 13.5; Pax3tm4Buck/Pax3tm4Buck (more )
Note: Triple labeled: green - Pax3 reporter; red - Cd34 (assay MGI:6511026); blue - alpha-SMA (Acta2 assay MGI:6511031).
Structure Level Pattern Image Note
TS21: forelimb Absent 4C Authors report total absence of myogenic cells in the forelimb.
Specimen 5A Flk1LacZ/+: embryonic day 9.0; Kdrtm1Jrt/Kdr+, Pax3tm4Buck/Pax3tm4Buck (more )
Structure Level Pattern Image Note
TS14: embryo Present 5A LacZ/+
Specimen 5A Flk1LacZ/LacZ: embryonic day 9.0; Kdrtm1Jrt/Kdrtm1Jrt, Pax3tm4Buck/Pax3tm4Buck (more )
Structure Level Pattern Image Note
TS14: embryo Present 5A LacZ/LacZ Authors state Flk1 (Kdr) knockout embryos are smaller than littermates.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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