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Gene Expression Data
Assay Details
Assay
Reference: J:156174 Ralston A, et al., Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2. Development. 2010 Feb;137(3):395-403
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6510807
Gene symbol: Cdx2
Gene name: caudal type homeobox 2
Antibody: anti- Cdx2 (CDX2-88)
Assay notes: Two antibodies were described: MGI:3051329 and MGI:5470245. The one used in this assay was not detailed.
Results
Specimen 4A: embryonic day 2.5 (more )
Note: Note: cytoplasmic staining detectable was likely to be background as it was present in Cdx2 mutants (not shown).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 4A Cdx2
Specimen 4B: embryonic day 3.0 (more )
Note: 16-cell
Structure Level Pattern Image Note
TS3: compacted morula Present 4B Cdx2
Specimen 4C: embryonic day 3.0 (more )
Note: 32-cell
Structure Level Pattern Image Note
TS4: compacted morula Present 4C Cdx2
Specimen 4D: embryonic day 3.5 (more )
Note: Blastocyst
Structure Level Pattern Image Note
TS4: trophectoderm Present Regionally restricted 4D Cdx2 Expression became restricted to outside cells of blastocyst in the nascent trophectoderm.
Specimen 6C: embryonic day 3.5 (more )
Note: Blastocyst
Structure Level Pattern Image Note
TS4: trophectoderm Present 6C Cdx2
Specimen 6D: embryonic day 3.5; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: Blastocyst
Structure Level Pattern Image Note
TS4: trophectoderm Absent 6D Cdx2
Specimen 7C: embryonic day 3.5; Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Structure Level Pattern Image Note
TS4: trophectoderm Present 7C Cdx2 , 7C Cdx2/Nuclei Cdx2 expression pattern was largely unaffected in Pou5f1 mutants.
TS4: inner cell mass Absent 7C Cdx2 , 7C Cdx2/Nuclei
Specimen 7D: embryonic day 4.25 (more )
Note: Implanting blastocyst.
Structure Level Pattern Image Note
TS6: epiblast Absent 7D Cdx2 , 7D Cdx2/Nuclei Expression was excluded from nuclei.
TS6: primitive endoderm Absent 7D Cdx2 , 7D Cdx2/Nuclei Expression was excluded from nuclei.
Specimen 7E: embryonic day 4.25; Pou5f1tm1.1Scho/Pou5f1tm1.1Scho (more )
Note: Implanting blastocyst.
Structure Level Pattern Image Note
TS6: epiblast Present 7E Cdx2/Nuclei , 7E Cdx2 Nuclear expression of Cdx2 was upregulated in the mutant epiblast and primitive endoderm.
TS6: primitive endoderm Present 7E Cdx2/Nuclei , 7E Cdx2 Nuclear expression of Cdx2 was upregulated in the mutant epiblast and primitive endoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory