Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54582
Iacopetti P, et al., Expression of the antiproliferative gene TIS21 at the onset of neurogenesis identifies single neuroepithelial cells that switch from proliferative to neuron-generating division. Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4639-44
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6508684
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Gene symbol:
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Btg2
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Gene name:
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BTG anti-proliferation factor 2
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Antibody:
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K4
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
1Da:
embryonic day 11.0
(more  )
Specimen
1Da:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: hindbrain |
Present |
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1Da
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Indistinguishable staining patterns compared with using the 1511 antibody.
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Specimen
not shown:
embryonic day 11.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
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Indistinguishable staining patterns compared with using the 1511 antibody.
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Specimen
not shown:
embryonic day 10.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: central nervous system |
Present |
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Indistinguishable staining patterns compared with using the 1511 antibody.
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Specimen
not shown:
embryonic day 12.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: central nervous system |
Present |
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Indistinguishable staining patterns compared with using the 1511 antibody.
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Specimen
4Aa:
embryonic day 10.0
(more )
Note: Also stained for Tubb3 (assay MGI:6508698).
Specimen
4Aa:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Also stained for Tubb3 (assay MGI:6508698).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future midbrain |
Present |
Regionally restricted |
4Aa
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Expressed at very low levels dorsally and more abundantly in the ventral region. Not only detected in the neuroepithelium, but also in neurons that had migrated to the mantle zone.
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Specimen
4Ba:
embryonic day 10.0
(more )
Note: Also stained for Tubb3 (assay MGI:6508698).
Specimen
4Ba:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
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Also stained for Tubb3 (assay MGI:6508698).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future spinal cord |
Present |
Regionally restricted |
4Ba
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Expressed in many cells in the neuroepithelium (with a ventrodorsal gradient) and the neurons in the mantle zone.
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| TS16: neural tube floor plate |
Present |
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4Ba
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Positive staining in area where lacks neurons (Tubb3-).
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Specimen
4Bc:
embryonic day 10.0
(more )
Note: Also stained for Tubb3 (assay MGI:6508698).
Specimen
4Bc:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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Also stained for Tubb3 (assay MGI:6508698).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future spinal cord |
Present |
Regionally restricted |
4Bc
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Expressed in many cells in the neuroepithelium (with a ventrodorsal gradient) and the neurons in the mantle zone.
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Specimen
4Be:
embryonic day 10.0
(more )
Note: Also stained for Tubb3 (assay MGI:6508698).
Specimen
4Be:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
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Also stained for Tubb3 (assay MGI:6508698).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future spinal cord |
Present |
Regionally restricted |
4Be
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Expressed in many cells in the neuroepithelium (with a ventrodorsal gradient) and the neurons in the mantle zone.
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Specimen
6A:
embryonic day 10.0
(more )
Specimen
6A:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future spinal cord |
Present |
Regionally restricted |
6A
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Barely expressed in the perikaryon and preferentially localized in the processes in newborn neurons in the ventricular zone and the neurons accumulating in the mantle zone (some were Tubb3-).
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| TS16: neural tube floor plate |
Present |
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6A
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Positive staining in area where lacks neurons (Tubb3-).
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Specimen
7A:
embryonic day 10.0
(more )
Specimen
7A:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7C/D:
embryonic day 10.0
(more )
Note: Double labeled: red - Btg2; green - anti-mitotic proteins antibody.
Specimen
7C/D:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Btg2; green - anti-mitotic proteins antibody.
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Specimen
7F:
embryonic day 10.0
(more )
Note: Double labeled: red - Btg2; green - anti-BrdU antibody.
Specimen
7F:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Btg2; green - anti-BrdU antibody.
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Specimen
7G:
embryonic day 10.0
(more )
Note: Double labeled: red - Btg2; green - anti-BrdU antibody.
Specimen
7G:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Btg2; green - anti-BrdU antibody.
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Analysis Tools