Probe:	Notch1 cDNA18
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS21: internal carotid artery	Present	Regionally restricted	3A	Expressed in the vascular smooth muscle cells.
TS21: pituitary gland	Absent		3A

Structure	Level	Pattern	Image	Note
TS21: internal carotid artery	Present	Regionally restricted	3E	Expressed in the vascular smooth muscle cells, same as in the wild-type.
TS21: pituitary gland	Absent		3E

Structure	Level	Pattern	Image	Note
TS21: developing vasculature of testis	Present		3I
TS21: interstitium of the testis	Present		3I

Structure	Level	Pattern	Image	Note
TS21: developing vasculature of testis	Present		3M	Same as in the wild-type.
TS21: interstitium of the testis	Present		3M	Same as in the wild-type.

Structure	Level	Pattern	Image	Note
TS21: hindlimb muscle	Present		3Q

Structure	Level	Pattern	Image	Note
TS21: hindlimb muscle	Present		3U	Same as in the wild-type.

Structure	Level	Pattern	Image	Note
TS21: midbrain roof plate	Present	Regionally restricted	4A	Expressed in the optic tectum.

Structure	Level	Pattern	Image	Note
TS21: abdomen musculature	Present
TS21: duodenum epithelium	Present
TS21: duodenum mesenchyme	Present
TS21: genital tubercle epithelium	Present
TS21: genital tubercle mesenchyme	Present
TS21: hyaloid vascular plexus	Present
TS21: hypothalamus	Present
TS21: lens anterior epithelium	Present
TS21: lobar bronchus epithelium	Present
TS21: lung mesenchyme	Present
TS21: medulla oblongata	Present
TS21: mesenchyme of metanephros	Present
TS21: nephric duct	Present
TS21: olfactory epithelium	Present
TS21: pancreas	Present
TS21: pleuro-peritoneal membrane	Present
TS21: renal tubule	Present
TS21: semicircular canal	Present
TS21: spinal cord ventricular layer	Present
TS21: stomach mesenchyme	Present
TS21: tongue	Present
TS21: neural retina	Present	Regionally restricted		Expressed in the central neural retina, not in the ciliary margin.
TS21: stomach	Present	Regionally restricted		Expressed in the mucosal lining.
TS21: back musculature	Present	Regionally restricted		Expressed in the erector spinae muscle.

Structure	Level	Pattern	Image	Note
TS21: abdomen musculature	Present			Same as in the wild-type.
TS21: duodenum epithelium	Present			Same as in the wild-type.
TS21: duodenum mesenchyme	Present			Same as in the wild-type.
TS21: genital tubercle epithelium	Present			Same as in the wild-type.
TS21: genital tubercle mesenchyme	Present			Same as in the wild-type.
TS21: hyaloid vascular plexus	Present			Same as in the wild-type.
TS21: hypothalamus	Present			Same as in the wild-type.
TS21: lens anterior epithelium	Present			Same as in the wild-type.
TS21: lobar bronchus epithelium	Present			Same as in the wild-type.
TS21: lung mesenchyme	Present			Same as in the wild-type.
TS21: medulla oblongata	Present			Same as in the wild-type.
TS21: mesenchyme of metanephros	Present			Same as in the wild-type.
TS21: nephric duct	Present			Same as in the wild-type.
TS21: olfactory epithelium	Present			Same as in the wild-type.
TS21: pancreas	Present			Same as in the wild-type.
TS21: pleuro-peritoneal membrane	Present			Same as in the wild-type.
TS21: renal tubule	Present			Same as in the wild-type.
TS21: spinal cord ventricular layer	Present			Same as in the wild-type.
TS21: stomach mesenchyme	Present			Same as in the wild-type.
TS21: neural retina	Present	Regionally restricted		Same as in the wild-type. Expressed in the central neural retina, not in the ciliary margin.
TS21: stomach	Present	Regionally restricted		Same as in the wild-type. Expressed in the mucosal lining.
TS21: back musculature	Present	Regionally restricted		Same as in the wild-type. Expressed in the erector spinae muscle.