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Gene Expression Data
Assay Details
Assay
Reference: J:217439 Srinivasan RS, et al., The Prox1-Vegfr3 feedback loop maintains the identity and the number of lymphatic endothelial cell progenitors. Genes Dev. 2014 Oct 1;28(19):2175-87
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6507015
Gene symbol: Prox1
Gene name: prospero homeobox 1
Antibody: anti-Prox1
Results
Specimen 2D: embryonic day 11.5; Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6507016); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: cardinal vein Weak Regionally restricted 2D Authors report Prox1 expression in lymphatic endothelial cell (LEC) progenitors reduced compared with wild-type littermates.
Specimen 2E: embryonic day 11.5; Flt4tm1Ali/Flt4+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6507016); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: cardinal vein Trace Regionally restricted 2E Prox1 expression in lymphatic endothelial cell (LEC) progenitors further reduced compared with Prox1+/GFPCre littermates.
Specimen 2G: embryonic day 13.5; Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6507016); blue - DAPI.
Structure Level Pattern Image Note
TS21: lymph sac Present 2G
Specimen 2H: embryonic day 13.5; Flt4tm1Ali/Flt4+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6507016); blue - DAPI.
Structure Level Pattern Image Note
TS21: lymphatic system Trace 2H Few Prox1-expressing lymphatic vessels.
Specimen 2I: embryonic day 15.5; Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6507016); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS23: lymphatic system Present Regionally restricted 2I Authors report mostly normal-looking lymphatic vessels in skin.
TS23: skin Present Regionally restricted 2I Authors report mostly normal-looking lymphatic vessels in skin.
Specimen 2J: embryonic day 15.5; Flt4tm1Ali/Flt4+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Pdpn (assay MGI:6507016); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS23: lymphatic system Trace 2J Few Prox1-expressing lymphatic vessels.
TS23: skin Trace 2J Few Prox1-expressing lymphatic vessels.
Specimen S2E: embryonic day 15.5; Flt4tm1Ali/Flt4+ (more )
Note: Triple labeled: green - Prox1; red - Vegfr3 (Flt4 assay MGI:6507018); blue - Lyve1 (assay MGI:6507020).
Structure Level Pattern Image Note
TS23: lymphatic system Present Regionally restricted S2E
TS23: skin Present Regionally restricted S2E
Specimen S2F: embryonic day 15.5; Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: green - Prox1; red - Vegfr3 (Flt4 assay MGI:6507018); blue - Lyve1 (assay MGI:6507020).
Structure Level Pattern Image Note
TS23: lymphatic system Present Regionally restricted S2F Authors report mostly normal-looking lymphatic vessels.
TS23: skin Present Regionally restricted S2F Authors report mostly normal-looking lymphatic vessels.
Specimen S2G: embryonic day 15.5; Flt4tm1Ali/Flt4+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: green - Prox1; red - Vegfr3 (Flt4 assay MGI:6507018); blue - Lyve1 (assay MGI:6507020).
Structure Level Pattern Image Note
TS23: lymphatic system Trace Regionally restricted S2G Lymphatic vessels were abnormally shaped or reduced in size and thinner. Authors report complete lack of lymphatic vessels in some double-heterozygous embryos.
TS23: skin Trace Regionally restricted S2G Lymphatic vessels were abnormally shaped or reduced in size and thinner. Authors report complete lack of lymphatic vessels in some double-heterozygous embryos.
Specimen 3A/C: embryonic day 10.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Conditional mutant. Triple labeled: red - Prox1; green - Cre reporter; blue - DAPI.
Structure Level Pattern Image Note
TS17: cardinal vein Present Regionally restricted 3C , 3A Numerous Prox1-expressing cells on cardinal vein.
Specimen 3D/F: embryonic day 10.5; Flt4tm1Ali/Flt4+, Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Conditional mutant. Triple labeled: red - Prox1; green - Cre reporter; blue - DAPI.
Structure Level Pattern Image Note
TS17: cardinal vein Weak Regionally restricted 3F , 3D Fewer Prox1-expressing cells on cardinal vein; most were GFP+.
Specimen 5A: embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: cardinal vein Present Regionally restricted 5A
Specimen 5A': embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: cardinal vein Present Regionally restricted 5A'
Specimen 5B: embryonic day 11.5; Flt4tm1Ali/Flt4+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: cardinal vein Present Regionally restricted 5B
Specimen 5B': embryonic day 11.5; Flt4tm1Ali/Flt4+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: cardinal vein Present Regionally restricted 5B'
Specimen 5C: embryonic day 11.5; Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: cardinal vein Present Regionally restricted 5C
Specimen 5C': embryonic day 11.5; Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: cardinal vein Present Regionally restricted 5C'
Specimen 5D: embryonic day 11.5; Flt4tm1Ali/Flt4+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: cardinal vein Weak Regionally restricted 5D Expression significantly reduced compared with single heterozygous littermates
Specimen 5D': embryonic day 11.5; Flt4tm1Ali/Flt4+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: cardinal vein Weak Regionally restricted 5D' Expression significantly reduced compared with single heterozygous littermates
Specimen 5E: embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted 5E
Specimen 5E': embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted 5E'
Specimen 5F: embryonic day 11.5; Flt4tm1Ali/Flt4+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted 5F
Specimen 5F': embryonic day 11.5; Flt4tm1Ali/Flt4+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted 5F'
Specimen 5G: embryonic day 11.5; Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted 5G
Specimen 5G': embryonic day 11.5; Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted 5G'
Specimen 5H: embryonic day 11.5; Flt4tm1Ali/Flt4+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: lymphatic system Weak Regionally restricted 5H Expression significantly reduced compared with single heterozygous littermates
Specimen 5H': embryonic day 11.5; Flt4tm1Ali/Flt4+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Vegfr3 (Flt4 assay MGI:6507018); blue - Pecam1 (assay MGI:6507017).
Structure Level Pattern Image Note
TS19: lymphatic system Weak Regionally restricted 5H' Expression significantly reduced compared with single heterozygous littermates
Specimen S3A: embryonic day 10.5; Vegfctm1Ali/Vegfc+ (more )
Note: Triple labeled: red - Prox1; green - Pecam1 (assay MGI:6507017); blue - Lyve1 (assay MGI:6507020).
Structure Level Pattern Image Note
TS17: cardinal vein Present Regionally restricted S3A Expression in numerous lymphatic endothelial cells (LECs) lining the anterior cardinal vein and budding from the vein.
TS17: lymphatic system Present Regionally restricted S3A Expression in numerous lymphatic endothelial cells (LECs) lining the anterior cardinal vein and budding from the vein.
Specimen S3B: embryonic day 10.5; Vegfctm1Ali/Vegfctm1Ali (more )
Note: Triple labeled: red - Prox1; green - Pecam1 (assay MGI:6507017); blue - Lyve1 (assay MGI:6507020).
Structure Level Pattern Image Note
TS17: cardinal vein Trace Regionally restricted S3B Severely reduced number of expressing cells inside the cardinal vein; almost none outside the vein.
TS17: lymphatic system Trace Regionally restricted S3B Severely reduced number of expressing cells inside the cardinal vein; almost none outside the vein.
Specimen 7A: embryonic day 11.5 (more )
Note: Triple labeled: red - Prox1; green - Pecam1 (assay MGI:6507017); blue - Pdpn (assay MGI:6507016).
Structure Level Pattern Image Note
TS19: cardinal vein Present Regionally restricted 7A
TS19: lymphatic system Present Regionally restricted 7A
Specimen 7B: embryonic day 11.5; Vegfctm1Ali/Vegfc+ (more )
Note: Triple labeled: red - Prox1; green - Pecam1 (assay MGI:6507017); blue - Pdpn (assay MGI:6507016).
Structure Level Pattern Image Note
TS19: cardinal vein Weak Regionally restricted 7B Expression in fewer lymphatic endothelial cell (LEC) progenitors and LECs compared with wild-type.
TS19: lymphatic system Weak Regionally restricted 7B Expression in fewer lymphatic endothelial cell (LEC) progenitors and LECs compared with wild-type.
Specimen 7C: embryonic day 11.5; Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Triple labeled: red - Prox1; green - Pecam1 (assay MGI:6507017); blue - Pdpn (assay MGI:6507016).
Structure Level Pattern Image Note
TS19: cardinal vein Weak Regionally restricted 7C Expression in fewer lymphatic endothelial cell (LEC) progenitors and LECs compared with wild-type.
TS19: lymphatic system Weak Regionally restricted 7C Expression in fewer lymphatic endothelial cell (LEC) progenitors and LECs compared with wild-type.
Specimen 7D: embryonic day 11.5; Prox1tm5(GFP/cre)Gco/Prox1+, Vegfctm1Ali/Vegfc+ (more )
Note: Triple labeled: red - Prox1; green - Pecam1 (assay MGI:6507017); blue - Pdpn (assay MGI:6507016).
Structure Level Pattern Image Note
TS19: cardinal vein Trace Regionally restricted 7D Expression in even fewer lymphatic endothelial cell (LEC) progenitors and LECs compared with single heterozygous littermates.
TS19: lymphatic system Trace Regionally restricted 7D Expression in even fewer lymphatic endothelial cell (LEC) progenitors and LECs compared with single heterozygous littermates.
Specimen S4A/C: embryonic day 11.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Prox1tm5(GFP/cre)Gco/Prox1+ (more )
Note: Conditional mutant. Triple labeled: red - Prox1; green - Cre reporter; blue - DAPI.
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted S4C , S4A
Specimen S4D/F: embryonic day 11.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Prox1tm5(GFP/cre)Gco/Prox1+, Vegfctm1Ali/Vegfc+ (more )
Note: Conditional mutant. Triple labeled: red - Prox1; green - Cre reporter; blue - DAPI.
Structure Level Pattern Image Note
TS19: lymphatic system Present Regionally restricted S4D , S4F
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