Gene Expression Data
Assay Details
Assay
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Reference:
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J:296649
Lindstrom NO, et al., Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning. J Am Soc Nephrol. 2018 Mar;29(3):825-840
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6505962
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Gene symbol:
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Krt8
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Gene name:
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keratin 8
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Antibody:
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TROMA 1
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
1B:
embryonic day 15.5
(more )
Note: Triple labeled: blue - Krt8; green - Lef1 (assay MGI:6505980); red - Six2 (assay MGI:6505986)
Specimen
1B:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Krt8; green - Lef1 (assay MGI:6505980); red - Six2 (assay MGI:6505986)
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: collecting duct |
Present |
|
1B left
|
Expression is seen in the ureteric bud.
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Specimen
1D'/S3B':
embryonic day 15.5
(more )
Note: Triple labeled: blue - Krt8; green - Pax8 (assay MGI:6505985); red - Six2 (assay MGI:6505986)
Specimen
1D'/S3B':
(close )
Genetic Background:
|
Swiss Webster
|
Age:
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embryonic day 15.5
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Triple labeled: blue - Krt8; green - Pax8 (assay MGI:6505985); red - Six2 (assay MGI:6505986)
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: collecting duct |
Present |
|
S3B'
,
1D'
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Expression is seen in the ureteric bud.
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Specimen
2A':
embryonic day 15.5
(more )
Note: Triple labeled: blue - Krt8; green - Foxc2 (assay MGI:6505977); red - Hnf1b (assay MGI:6505983)
Specimen
2A':
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: blue - Krt8; green - Foxc2 (assay MGI:6505977); red - Hnf1b (assay MGI:6505983)
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: collecting duct |
Present |
|
2A'
|
Expression is seen in the ureteric bud.
|
Specimen
2C':
embryonic day 15.5
(more )
Note: Triple labeled: blue - Krt8; green - Lef1 (assay MGI:6505980); red - Six2 (assay MGI:6505986)
Specimen
2C':
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: blue - Krt8; green - Lef1 (assay MGI:6505980); red - Six2 (assay MGI:6505986)
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: collecting duct |
Present |
|
2C'
|
Expression is seen in the ureteric bud.
|
Specimen
2D':
embryonic day 15.5
(more )
Note: Multi-labeled: blue - Krt8; green - Sox9 (assay MGI:6505981); red - Mafb (assay MGI:6505978); purple - Jag1 (assay MGI:6505988)
Specimen
2D':
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Multi-labeled: blue - Krt8; green - Sox9 (assay MGI:6505981); red - Mafb (assay MGI:6505978); purple - Jag1 (assay MGI:6505988)
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Specimen
S1B':
embryonic day 15.5
(more )
Note: Triple labeled: blue - Krt8; green - Sox9 (assay MGI:6505981); red - Sall1 (assay MGI:6505991)
Specimen
S1B':
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: blue - Krt8; green - Sox9 (assay MGI:6505981); red - Sall1 (assay MGI:6505991)
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: collecting duct |
Present |
|
S1B'
|
Expression is seen in the ureteric bud.
|
Specimen
S1C':
embryonic day 15.5
(more )
Note: Triple labeled: blue - Krt8; green - Pax8 (assay MGI:6505985); red - Six2 (assay MGI:6505986)
Specimen
S1C':
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: blue - Krt8; green - Pax8 (assay MGI:6505985); red - Six2 (assay MGI:6505986)
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: collecting duct |
Present |
|
S1C'
,
S1C' SIX2/KRT8
|
Expression is seen in the ureteric bud.
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Specimen
S2 upper:
postnatal day 2
(more )
Note: Triple labeled: blue - Krt8; green - Six2 (assay MGI:6505986); red - Lef1 (assay MGI:6505980)
Specimen
S2 upper:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: blue - Krt8; green - Six2 (assay MGI:6505986); red - Lef1 (assay MGI:6505980)
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: collecting duct |
Present |
|
S2_upper
|
Expression is seen in the ureteric bud.
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Specimen
S2 lower:
postnatal day 2
(more )
Note: Triple labeled: blue - Krt8; green - Six2 (assay MGI:6505986); red - Lef1 (assay MGI:6505980)
Specimen
S2 lower:
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
postnatal day 2
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: blue - Krt8; green - Six2 (assay MGI:6505986); red - Lef1 (assay MGI:6505980)
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: collecting duct |
Present |
|
S2_lower
|
Expression is seen in the ureteric bud.
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