Gene Expression Data
Assay Details
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Assay
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Reference:
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J:36690
Buck CA, et al., Expression patterns of adhesion receptors in the developing mouse lung: functional implications. Cell Adhes Commun. 1996 Aug;4(2):69-87
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6504910
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Gene symbol:
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Icam1
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Gene name:
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intercellular adhesion molecule 1
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Antibody:
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YN-1
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Embryos were staged according to Kaufman, M.H. (1992) The Atlas of Mouse Development. Academic Press, London.
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Results
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Specimen
5A:
embryonic day 12.5
(more  )
Note: Double labeled: green - Icam1; red - laminin.
Specimen
5A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Icam1; red - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lung mesenchyme |
Weak |
Regionally restricted |
5A
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Expression is in the extra bronchial mesenchyme.
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Specimen
5B:
embryonic day 16.5
(more )
Note: Double labeled: green - Icam1; red - laminin.
Specimen
5B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Icam1; red - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: bronchiole epithelium |
Present |
Regionally restricted |
5B
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Expression is in forming airways on epithelial cells just distal to the regions of dichotomous branching and is absent in the more proximal bronchiolal or bornochial epithelium.
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| TS24: lobar bronchus epithelium |
Present |
Regionally restricted |
5B
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Expression is in forming airways on epithelial cells just distal to the regions of dichotomous branching and is absent in the more proximal bronchiolal or bornochial epithelium.
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| TS24: lung epithelium |
Present |
Regionally restricted |
5B
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Expression is in forming airways on epithelial cells just distal to the regions of dichotomous branching and is absent in the more proximal bronchiolal or bornochial epithelium.
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| TS24: respiratory bronchiole epithelium |
Present |
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5B
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Specimen
5C:
embryonic day 18.0
(more )
Note: Double labeled: green - Icam1; red - laminin.
Specimen
5C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Icam1; red - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: bronchiole epithelium |
Strong |
Regionally restricted |
5C
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Expression is in pre-acinar bronchioles.
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| TS26: lung epithelium |
Present |
Regionally restricted |
5C
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Expression is in forming airways on epithelial cells just distal to the regions of dicotomous branching and is absent in larger airways.
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| TS26: lobar bronchus |
Absent |
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5C
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Specimen
5D:
postnatal day 0
(more )
Note: Double labeled: green - Icam1; red - laminin.
Specimen
5D:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Icam1; red - laminin.
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Specimen
5E:
postnatal day 17
(more )
Specimen
5E:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 17
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Specimen
5F:
postnatal day 30
(more )
Note: Double labeled: green - Icam1; red - laminin.
Specimen
5F:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 30
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Icam1; red - laminin.
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