Gene Expression Data
Assay Details
Assay
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Reference:
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J:135108
Manning JA, et al., Nedd1 expression as a marker of dynamic centrosomal localization during mouse embryonic development. Histochem Cell Biol. 2008 Jun;129(6):751-64
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6504011
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Gene symbol:
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Tubg1
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Gene name:
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tubulin, gamma 1
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Antibody:
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anti-gamma-tubulin (GTU-88, T6557)
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Assay notes:
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The specificity of the antibody used was not detailed, both family members have been annotated (Tubg1 and Tubg2).
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Results
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Specimen
4a:
embryonic day 11.5
(more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Specimen
4a:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
|
Specimen
4b:
embryonic day 11.5
(more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Specimen
4b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
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embryonic day 11.5
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
|
Note:
|
Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
|
Specimen
4c:
embryonic day 11.5
(more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Specimen
4c:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
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Embedding:
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Cryosection
|
Note:
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Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube ventricular layer |
Present |
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4c Merge
,
4c Nedd1/g-tubulin
,
4c g-tubulin
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Expression is in centrosomes.
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TS19: dorsal root ganglion |
Present |
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4c g-tubulin
,
4c Merge
,
4c Nedd1/g-tubulin
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Expression is as a centrosomal spot or as weak difffuse cytoplasmic staining.
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Specimen
5a:
embryonic day 11.5
(more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Specimen
5a:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
Methanol
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: lens vesicle epithelium |
Present |
Regionally restricted |
5a Nedd1/g-tubulin
,
5a Merge
,
5a g-tubulin
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Expression is centrosomes in the apical region of polarized epithelial cells.
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Specimen
5b:
embryonic day 11.5
(more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Specimen
5b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Methanol
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural retina |
Present |
Regionally restricted |
5b Nedd1/g-tubulin
,
5b g-tubulin
,
5b Merge
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Expression is at the subventricular region of polarized invaginated neural ectoderm cells.
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Specimen
5c:
embryonic day 14.5
(more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Specimen
5c:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Methanol
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lens epithelium |
Present |
Regionally restricted |
5c g-tubulin
,
5c Merge
,
5c Nedd1/g-tubulin
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There is an apical polarized expression.
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Specimen
5d:
embryonic day 14.5
(more )
Note: Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
Specimen
5d:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Methanol
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Tubg1 and Tubg2; green - Nedd1 (assay MGI:6503997).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: neural retina |
Present |
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5d Nedd1/g-tubulin
,
5d g-tubulin
,
5d Merge
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Expression is in a polarized pattern in the subventricular region of cells of the outer neuroblastic layer.
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