Cdh1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:289397 Kowalkowski A, et al., Lack of discreet colocalization of epithelial apoptosis to the atretic precursor in the colon of the Fibroblast growth factor receptor 2IIIb mouse and staining consistent with cellular movement suggest a revised model of atresia formation. Dev Dyn. 2020 Jun;249(6):741-753
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6491431
Gene symbol:	Cdh1
Gene name:	cadherin 1

Antibody:	Anti-E-Cadherin (610181; Clone 36)
Detection system:	Alexa Fluor 488
Assay notes:	Fgfr2IIIb-/- (null) embryos were generated by mating Fgfr2IIIbflox/+; HprtCre/+ females with Fgfr2IIIbflox/flox males. Fgfr2IIIbflox/flox (control) embryos were generated within the same litters without the HPRT-Cre allele.

Results

7A ©

Specimen 7A: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn (more

)
Note: Triple labeled: green - Cdh1; red - laminin; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS19: large intestine	Present	Regionally restricted	7A	Expression in proximal colon.

7B ©

Specimen 7B: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn (more

)
Note: Triple labeled: green - Cdh1; red - laminin; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS19: large intestine	Present	Regionally restricted	7B	Expression in distal colon.

7C ©

Specimen 7C: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn, Hprt1^{tm1(CAG-cre)Mnn}/Hprt1⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Cdh1; red - laminin; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS19: large intestine	Present	Regionally restricted	7C	Expression in proximal colon.

7D ©

Specimen 7D: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn, Hprt1^{tm1(CAG-cre)Mnn}/Hprt1⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Cdh1; red - laminin; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS19: large intestine	Present	Regionally restricted	7D	Expression in atretic precursor region.

8A ©

Specimen 8A: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn (more

)
Note: Double labeled: green - Cdh1; red - Ctnnb1 (assay MGI:6491432).

Structure	Level	Pattern	Image	Note
TS19: large intestine	Present	Regionally restricted	8A	Expression in colon epithelial cells.
TS19: small intestine epithelium	Present		8A

8B ©

Specimen 8B: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn, Hprt1^{tm1(CAG-cre)Mnn}/Hprt1⁺ (more

)
Note: Conditional mutant. Double labeled: green - Cdh1; red - Ctnnb1 (assay MGI:6491432).

Structure	Level	Pattern	Image	Note
TS19: large intestine	Present	Regionally restricted	8B	Expression in colon epithelial cells.

8C ©

Specimen 8C: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn (more

)
Note: Double labeled: green - Cdh1; red - Acta2 (assay MGI:6491433).

Structure	Level	Pattern	Image	Note
TS19: large intestine	Present	Regionally restricted	8C	Expression in colon epithelial cells.
TS19: small intestine epithelium	Present		8C

8D ©

Specimen 8D: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn, Hprt1^{tm1(CAG-cre)Mnn}/Hprt1⁺ (more

)
Note: Conditional mutant. Double labeled: green - Cdh1; red - Acta2 (assay MGI:6491433).

Structure	Level	Pattern	Image	Note
TS19: large intestine	Present	Regionally restricted	8D	Expression in colon epithelial cells.
TS19: small intestine epithelium	Present		8D

8E ©

Specimen 8E: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn (more

)
Note: Double labeled: green - Cdh1; red - Vim (assay MGI:6491434).

Structure	Level	Pattern	Image	Note
TS19: large intestine	Present	Regionally restricted	8E	Expression in colon epithelial cells.
TS19: small intestine epithelium	Present		8E

8F ©

Specimen 8F: embryonic day 11.5; Fgfr2^tm1Dsn/Fgfr2^tm1Dsn, Hprt1^{tm1(CAG-cre)Mnn}/Hprt1⁺ (more

)
Note: Conditional mutant. Double labeled: green - Cdh1; red - Vim (assay MGI:6491434).

Structure	Level	Pattern	Image	Note
TS19: large intestine	Present	Regionally restricted	8F	Expression in colon epithelial cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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