Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Regionally restricted	1A	Expression throughout dermomyotome, with strongest expression in hypaxial epithelium. Expression in hypoglossal chord.
TS17: limb mesenchyme	Present	Regionally restricted	1A	Expression in muscle progenitor cells migrating to limbs.
TS17: neural tube	Present	Regionally restricted	1A	Expression in dorsal neural tube.
TS17: fronto-nasal process	Present		1A

Structure	Level	Pattern	Image	Note
TS17: somite	Weak	Regionally restricted	1B	Expression reduced in somites mainly in hypaxial, but also in epaxial domains, and somite segmentation is perturbed. Authors report lack of hypoglossal chord.
TS17: neural tube	Present	Regionally restricted	1B	Authors report failure of neural tube closure.
TS17: limb mesenchyme	Absent		1B	Authors report lack of migrating precursor cells to limbs.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Regionally restricted	1E	Expression in dorsal neural tube/
TS21: limb muscle	Present		1E
TS21: trunk musculature	Present		1E
TS21: craniocervical region muscle	Present	Regionally restricted	1E	Expression in cervical muscles.
TS21: cranial muscle	Absent		1E	No expression in head muscle.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Regionally restricted	1F	Authors report defects in neural tube closure, lack of limb muscles, and reduced and disorganized cervical and trunk muscles.
TS21: craniocervical region muscle	Present	Regionally restricted	1F	Authors report defects in neural tube closure, lack of limb muscles, and reduced and disorganized cervical and trunk muscles.
TS21: limb muscle	Present	Regionally restricted	1F	Authors report defects in neural tube closure, lack of limb muscles, and reduced and disorganized cervical and trunk muscles.
TS21: trunk musculature	Present	Regionally restricted	1F	Authors report defects in neural tube closure, lack of limb muscles, and reduced and disorganized cervical and trunk muscles.
TS21: cranial muscle	Absent		1F	No expression in head muscle.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	1R	Expression in dorsal neural tube.
TS19: cranial ganglion	Present		1R
TS19: dorsal root ganglion	Present		1R
TS19: mesenchyme derived from neural crest	Present		1R
TS19: somite	Present		1R

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present		3A	Expression in dorsal neural tube.
TS19: cranial ganglion	Present		3A
TS19: dorsal root ganglion	Present		3A
TS19: mesenchyme derived from neural crest	Present		3A
TS19: somite	Present		3A
TS19: forelimb bud mesenchyme	Present		3A

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	3E	Expression in dorsal neural tube.
TS19: cranial ganglion	Present		3E
TS19: dorsal root ganglion	Present		3E
TS19: mesenchyme derived from neural crest	Present		3E
TS19: somite	Present		3E
TS19: forelimb bud mesenchyme	Absent		3E	Forelimb muscles not present in nLacZ/nLacZ embryos.

Structure	Level	Pattern	Image	Note
TS19: thoracic segment of trunk	Present		3F

Structure	Level	Pattern	Image	Note
TS19: thoracic segment of trunk	Present		3J

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		3K
TS19: forelimb bud mesenchyme	Present		3K

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		3O
TS19: forelimb bud mesenchyme	Absent		3O	Forelimb muscles not present in nLacZ/nLacZ embryos.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	6E	At E10.25 (31 somites), migration to forelimb is virtually complete.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	6G	At E10.25 (31 somites), migration to forelimb is virtually complete
TS16: embryo mesenchyme	Present	Regionally restricted	6G	Expression in cells that will contribute to diaphragm.