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Gene Expression Data
Assay Details
Assay
Reference: J:231660 Punzo D, et al., Age-Related Changes in d-Aspartate Oxidase Promoter Methylation Control Extracellular d-Aspartate Levels and Prevent Precocious Cell Death during Brain Aging. J Neurosci. 2016 Mar 9;36(10):3064-78
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6478315
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-activated caspase-3
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: The antibody used recognizes the activated form of the protein.
Results
Specimen 4a 3 months: postnatal month 3 (more )
Structure Level Pattern Image Note
TS28: infralimbic area Present Single cells 4a 3 months Ddo+/+ left , 4a 3 months Ddo+/+ right Expression is in a few cells in the laminae II/III of the infralimbic cortex.
Specimen 4a 6 months: postnatal month 6 (more )
Structure Level Pattern Image Note
TS28: infralimbic area Present Single cells 4a 6 months Ddo+/+ right , 4a 6 months Ddo+/+ left Expression is in a few cells in the laminae II/III of the infralimbic cortex.
Specimen 4a 13 months: postnatal month 13 (more )
Structure Level Pattern Image Note
TS28: infralimbic area Present Single cells 4a 13 months Ddo+/+ left , 4a 13 months Ddo+/+ right Expression is in a few cells in the laminae II/III of the infralimbic cortex.
Specimen 4a Ddo-/- 3 months: postnatal month 3; Ddotm1Rdl/Ddotm1Rdl (more )
Structure Level Pattern Image Note
TS28: infralimbic area Present Single cells 4a 3 months Ddo-/- right , 4a 3 months Ddo-/- left Expression in this mutant is the same as in wild-type.
Specimen 4a Ddo-/- 6 months: postnatal month 6; Ddotm1Rdl/Ddotm1Rdl (more )
Structure Level Pattern Image Note
TS28: infralimbic area Present Single cells 4a 6 months Ddo-/- right , 4a 6 months Ddo-/- left There is a significantly increased percentage of activated caspase-3-positive cells in this mutant compared to wild-type.
Specimen 4a Ddo-/- 13 months: postnatal month 13; Ddotm1Rdl/Ddotm1Rdl (more )
Structure Level Pattern Image Note
TS28: infralimbic area Present Single cells 4a 13 months Ddo-/- left , 4a 13 months Ddo-/- right There is a significantly increased percentage of activated caspase-3-positive cells in this mutant compared to wild-type.
Specimen 4a NeuN/Casp +/+: postnatal month 13 (more )
Note: Double labeled: green - Casp3; purple - Rbfox3 (assay MGI:6478324).
Structure Level Pattern Image Note
TS28: infralimbic area Present Single cells 4a Merge Ddo+/+ Expression is in laminae II/III of the infralimbic cortex.
Specimen 4a NeuN/Casp -/-: postnatal month 13; Ddotm1Rdl/Ddotm1Rdl (more )
Note: Double labeled: green - Casp3; purple - Rbfox3 (assay MGI:6478324).
Structure Level Pattern Image Note
TS28: infralimbic area Present Single cells 4a Merge Ddo-/- The number of activated caspase-3-positive cells has increased in this mutant compared to wild-type.
Specimen 6a 3 months +/+: postnatal month 3 (more )
Note: Double labeled: green - Casp3; red - Th (assay MGI:6478319).
Structure Level Pattern Image Note
TS28: substantia nigra Present Single cells 6a 3 months Caspase-3 Ddo+/+ , 6a 3 months merge Ddo+/+
Specimen 6a 3 months -/-: postnatal month 3; Ddotm1Rdl/Ddotm1Rdl (more )
Note: Double labeled: green - Casp3; red - Th (assay MGI:6478319).
Structure Level Pattern Image Note
TS28: substantia nigra Present Single cells 6a 3 months merge Ddo-/- , 6a 3 months Caspase-3 Ddo-/- The number of activated caspase-3-positive cells has increased in this mutant compared to wild-type age-matched controls.
Specimen 6a 6 months +/+: postnatal month 6 (more )
Note: Double labeled: green - Casp3; red - Th (assay MGI:6478319).
Structure Level Pattern Image Note
TS28: substantia nigra Present Single cells 6a 6 months merge Ddo+/+ , 6a 6 months Caspase-3 Ddo+/+
Specimen 6a 6 months -/-: postnatal month 6; Ddotm1Rdl/Ddotm1Rdl (more )
Note: Double labeled: green - Casp3; red - Th (assay MGI:6478319).
Structure Level Pattern Image Note
TS28: substantia nigra Present Single cells 6a 6 months merge Ddo-/- , 6a 6 months Caspase-3 Ddo-/- The number of activated caspase-3-positive cells has increased in this mutant compared to wild-type age-matched controls.
Specimen 6a 13 months +/+: postnatal month 13 (more )
Note: Double labeled: green - Casp3; red - Th (assay MGI:6478319).
Structure Level Pattern Image Note
TS28: substantia nigra Present Single cells 6a 13 months merge Ddo+/+ , 6a 13 months Caspase-3 Ddo+/+
Specimen 6a 13 months -/-: postnatal month 13; Ddotm1Rdl/Ddotm1Rdl (more )
Note: Double labeled: green - Casp3; red - Th (assay MGI:6478319).
Structure Level Pattern Image Note
TS28: substantia nigra Present Single cells 6a 13 months Caspase-3 Ddo-/- , 6a 13 months merge Ddo-/- The number of activated caspase-3-positive cells has increased in this mutant compared to wild-type age-matched controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory